煤焦沥青烟提取物对人支气管上皮细胞的脂质过氧化作用
Lipid Peroxidation Induced by Coal Tar Pitch Smoke Extract in Human Bronchial Epithelial Cells
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摘要:
目的 研究煤焦沥青烟提取物(CTPE)刺激人支气管上皮细胞(BEAS-2B)的脂质过氧化作用。 方法 用不同浓度(0、1、3μg/mL)的CTPE刺激BEAS-2B细胞4 h后,流式细胞仪检测细胞活性氧(ROS)水平。刺激BEAS-2B细胞8 h后,用丙二醛(MDA)试剂盒和超氧化物歧化酶(SOD)活力测定试剂盒分别检测细胞裂解液中的MDA含量和SOD活力。 结果 与对照组相比,CTPE (1、3μg/mL)作用细胞4 h后,ROS含量均显著升高(P < 0.05)。1、3μg/mL CTPE刺激细胞8 h后,MDA含量均升高,SOD活力均明显降低(P < 0.05);MDA/SOD值升高(P < 0.05)。 结论 煤焦沥青烟提取物刺激BEAS-2B细胞后ROS水平和MDA含量增高,SOD活力降低,发生了脂质过氧化作用。 Abstract:Objective To explore whether coal tar pitch smoke extract (CTPE) could induce lipid peroxidation in human bronchial epithelial cells (BEAS-2B) Methods BEAS-2B cells were exposed to different concentrations (0,1,and 3 μg/mL) of CTPE for four hours and measured for the levels of reactive oxygen species (ROS) by flow cytometry.The amount of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in the cells exposed to CTPE for eight hours were analyzed using MDA and SOD assay kits,respectively. Results The levels of ROS in the BEAS-2B cells treated with CTPE (1 and 3μg/mL) for four hours were higher than that in the control group.After eight hours exposure,the activity of SOD in the CTPE groups (1 and 3μg/mL) was significantly decreased compared with that in the control group;similarly,the amount of MDA and the ratio of MDA/SOD were also significantly higher than those in the control group. Conclusion CTPE can elevate levels of ROS and MDA,while decrease SOD activity.These results indicate that CTPE might induce lipid peroxidation in BEAS-2B cells.
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