甲基叔丁基醚及其代谢物叔丁醇对人外周血淋巴细胞彗星尾矩的影响
Effects of Serum Methyl Tert-Butyl Ether and Related Metabolite Tert-Butyl Alcohol on Olive Tail Moment of Lymphocytes in Peripheral Blood
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摘要:
目的 探讨血清中汽油添加剂甲基叔丁基醚(MTBE)及其代谢产物叔丁醇(TBA)对人外周血淋巴细胞彗星尾矩的影响。 方法 以华南地区8家加油站作为研究现场,共抽取加油站从业人员100名作为研究对象进行流行病学横断面调查,各采集外周静脉血样5 mL,分离出血清和淋巴细胞;运用气相色谱-质谱(GC-MS)联用技术测定血清中MTBE及其代谢产物TBA含量(μg/L);运用彗星试验分析样本人群外周血淋巴细胞DNA彗星Olive尾矩。采用单因素和多因素非条件logistic回归分析方法,探讨样本人群外周血淋巴细胞DNA彗星Olive尾矩的主要影响因素。 结果 单因素logistic回归分析显示,血清MTBE含量(μg/L)、血清TBA含量(μg/L)、工种、饮酒情况、烟龄、平均每天吸烟支数及吸烟指数等7因素对外周血淋巴细胞DNA彗星Olive尾矩的影响具有统计学意义(P < 0.05);多因素logistic回归分析结果显示,血清MTBE和工种2因素最终引入回归方程,其OR值(95% CI)分别为4.155(2.279~7.577)和6.949(1.305~36.990),二者P值<0.05。 结论 MTBE暴露对人外周血淋巴细胞DNA彗星尾矩影响明显,可能具有遗传毒性。 Abstract:Objective To evaluate the effects of methyl tert-butyl ether (MTBE) and its metabolite tert-butyl alcohol (TBA) on comet tail moment of lymphocytes in human peripheral blood. Methods Employees (n=100) from eight gas stations were enrolled in an epidemiological cross-sectional study.Serum and lymphocytes were isolated from the peripheral venous blood sample (5 mL) of each subject,and analyzed for the content of serum MTBE and TBA using gas chromatograph-mass spectrometer (GC-MS) and the Olive tail moment (OTM) of lymphocytes by comet assay.Univariate and multivariate non-conditional logistic regression analyses were used to evaluate the effects of MTBE and TBA on OTM of human lymphocytes. Results The results of univariate logistic regression analysis showed that the contents of MTBE and TBA in serum,type of work,drinking,smoking,average number of cigarettes smoked per day,and smoking index,were associated with the OTM of lymphocytes (P < 0.05).The results of multivariate non-conditional logistic regression analysis showed that serum MTBE content and type of work were associated with the OTM of lymphocytes,and the values of odds ratio (OR) were 4.155(95% CI:2.279-7.577) and 6.949(1.305-36.990),respectively (both P < 0.05). Conclusion MTBE shows an obvious effect on the OTM of human peripheral blood lymphocytes,and may have genetic toxicity to human body.
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