李云路, 原福胜, 李敏, 梁瑞峰, 赵五红. 甲醛和三氯乙烯联合染毒对小鼠肾脏的氧化损伤作用[J]. 环境与职业医学, 2014, 31(2): 130-133. DOI: 10.13213/j.cnki.jeom.2014.0034
引用本文: 李云路, 原福胜, 李敏, 梁瑞峰, 赵五红. 甲醛和三氯乙烯联合染毒对小鼠肾脏的氧化损伤作用[J]. 环境与职业医学, 2014, 31(2): 130-133. DOI: 10.13213/j.cnki.jeom.2014.0034
LI Yun-lu , YUAN Fu-sheng , LI Min , LIANG Rui-feng , ZHAO Wu-hong . Renal Oxidative Damage Induced by Joint Exposure to Formaldehyde and Trichloroethylene inMice[J]. Journal of Environmental and Occupational Medicine, 2014, 31(2): 130-133. DOI: 10.13213/j.cnki.jeom.2014.0034
Citation: LI Yun-lu , YUAN Fu-sheng , LI Min , LIANG Rui-feng , ZHAO Wu-hong . Renal Oxidative Damage Induced by Joint Exposure to Formaldehyde and Trichloroethylene inMice[J]. Journal of Environmental and Occupational Medicine, 2014, 31(2): 130-133. DOI: 10.13213/j.cnki.jeom.2014.0034

甲醛和三氯乙烯联合染毒对小鼠肾脏的氧化损伤作用

Renal Oxidative Damage Induced by Joint Exposure to Formaldehyde and Trichloroethylene inMice

  • 摘要: 目的 探究甲醛和三氯乙烯联合染毒对小鼠肾脏的氧化损伤作用。

    方法 共108只清洁级昆明种小鼠,按3& #215;3析因设计随机平均分为9组:对照组(清洁空气)、甲醛低(1mg/m3)、甲醛高(5mg/m3)、三氯乙烯低(1000mg/m3)、三氯乙烯高(5 000 mg/m3)、甲醛低+三氯乙烯低(1 mg/m3+1 000 mg/m3)、甲醛低+三氯乙烯高(1 mg/m3+5 000 mg/m3)、甲醛高+三氯乙烯低(5 mg/m3+1 000 mg/m3)、甲醛高+三氯乙烯高(5 mg/m3+5 000 mg/m3)剂量组,每组12只,雌雄各半。采用静式吸入染毒,每天2 h,连续14 d。染毒结束后测定肾组织总抗氧化能力(total antioxidant capacity,T-AOC)、还原型谷胱甘肽(glutathione,GSH)、总超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)含量。

    结果 小鼠肾组织T-AOC、SOD和GSH随染毒剂量升高而明显降低,MDA含量则明显上升,差异均有统计学意义(P<0.05)。其中,联合染毒对雌性小鼠T-AOC的影响存在一定交互作用,具有统计学意义(P<0.05),其他各指标的交互作用并不显著。

    结论 甲醛和三氯乙烯吸入染毒对小鼠肾脏均具有氧化损伤作用,两者联合可能存在一定的交互作用。

     

    Abstract: Objective To examine the possible renal oxidative damage of joint exposure to formaldehyde andtrichloroethylene in mice.

    Methods Totally 108 healthy Kunming inbred strain mice were randomly divided into ninegroups,each with half male and half female:a control group (clean air),two formaldehyde treatment groups (1 mg/m3,5 mg/m3),twotrichloroethylene treatment groups (1 000 mg/m3,5 000 mg/m3),and four joint treatment groups of formaldehyde and trichloroethylene (1 mg/m3+1 000 mg/m3,1 mg/m3+5 000 mg/m3,5 mg/m3+1 000 mg/m3,5 mg/m3+5 000 mg/m3,respectively).The mice were exposedto formaldehyde and/or trichloroethylene (except the controls) by static inhalation for 14 days,2 hours per day,then neutralized onthe 15th day.Total antioxidant capacity (T-AOC),glutathione (GSH),superoxide dismutase (SOD),and malondialdehyde (MDA) in the renal samples of the mice were determined.

    Results The contents of GSH and the activities of T-AOC and SOD weredecreased,and the contents of MDA was increased along with the increasing of formaldehyde and/or trichloroethylene (P<0.05).There was an interaction between formaldehyde and trichloroethylene exposures on T-AOC of female mice (P<0.05).

    Conclusion Inhaling formaldehyde and/or trichloroethylene can induce oxidative damage in renal of mice.The joint exposure to formaldehydeand trichloroethylene may exhibit antagonistic effect on oxidative damage in renal.

     

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