Objective To observe transitional epithelial response gene1 (TERE1) in normal forelimbs development and retinoic acid-induced short limb malformations in mouse embryogenesis, and to explore the relationship between TERE1 and the short limbs.
Methods At gestational day 10 (GD10), the gestational mice of the treatment group were administered with 80 mg/kg all-trans retinoic acid (atRA), and those of the control group were administered with the same volume soybean oil. The forelimbs of all embryos were harvested during GD11-GD18. Mice embryonic forelimbs were explanted on GD12 and harvested after cultured for 24 h, 48 h and 72 h induced by atRA in various concentrations. The expressional abundance of TERE1 in all samples was measured by quantitative real-time polymerase chain reaction (QRT-PCR).
Results During the whole animal experiments, short limb malformations of mice embryos were induced apparently by atRA, and a variety of skeletal abnormalities of mice embryo forelimbs were also induced by atRA. TERE1 was expressed in all samples. The expression of TERE1 in the short limbs was lower than that in normal limbs on GD11-GD14 and GD16-GD18, but showed no difference between each other on GD15. In the limbs cultured in vitro, the expression of TERE1 was increased with the cultured times. In the treatment group, the expression that affected by different dose atRA was lower than that of the control group at cultured for 24 h (P < 0.05). At cultured for 24 h and 48 h, the expression of this gene increased with the increasing dose of atRA form 2.5& #215;10-6 mol/L to 1.0& #215;10-5 mol/L, but decreased in the highest dose of atRA (2.0& #215;10-5 mol/L) group.
Conclusion TERE1 may play a role during the development of the mice embryo forelimbs, and the mRNA expression of TERE1 changed in the forelimb malformations induced by atRA during mouse embryogenesis.
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