Objective To explore the protective effects of low intensity microwave on doxorubicin hydrochloride (DOX) induced damages in HL-60 cells.
Methods HL-60 cells were randomly divided into control,microwave alone,DOX alone,and combined exposure groups.The microwave alone and the combined exposure groups were exposed to 12 μW/cm2 microwaves,1 hour per day for 3 days.The cells in the control and the DOX alone groups were placed in the same condition,but without microwave radiation.The combined exposure and the DOX alone groups were subsequently exposed to DOX at the concentration of 0.125 mg/L on the 4th day.Apoptosis was detected with Annexin V-FITC/PI double staining.Mitochondrial membrane potential (MMP) and intracellular Ca2+concentrations were determined by flow cytometry.
Results Compared with the control group,the apoptosis rate and Ca2+concentration of the DOX alone group significantly increased (P<0.05),while the MMP statistically decreased (P<0.05).Compared with the DOX alone group,the apoptosis rate and Ca2+concentration of the combined exposure group significantly decreased (P<0.05),while the MMP statistically increased (P<0.05).
Conclusion Under this experimental condition,preexposure with 12 μW/cm2 900 MHz microwaves could significantly alleviate DOX-induced damages to HL-60 cells.
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