Detection of Multiple Serum Adipokines in Patients with Type 2 Diabetes Mellitus Using Multiplex Analysis Technology: Application and Advantages

Objective To detect the levels of multiple serum adipokines in elderly patients with type 2 diabetes and healthy controls by multiplex analysis technology.

Methods A total of 28 cases of elderly patients with type 2 diabetes mellitus (T2DM) and 22 cases of healthy controls were recruited in this study. Levels of serum multiple adipokines including adiponectin, resistin, adipsin, lipocalin-2, and plasminogen activator inhibitor-1 (PAI-1) were detected by the Magpix liquid chip instrument. The difference of the levels between the two groups was analyzed.

Results Only serum PAI-1 level of the T2DM group was 1.29 times that of the control group (P < 0.05), whereas other four adipokines had no differences (P>0.05).

Conclusion PAI-1 level is closely associated with glycometabolism. Multiplex analysis technology facilitates chronic disease biomonitoring and control.