Effect on Biological Function of Lung Cancer 95D Cells by MicroRNA-125b

Objective To investigate the effect of microRNA (miR)-125b on biological function of lung cancer 95D cells.

Methods The study transfected miR-125b mimics into 95D cells (mimics transfection group). A mimics negative control group and a blank group were also established. MTT assay, flow cytometry, and Transwell assay were used to examine the effects of up-regulated miR-125b on the proliferation, apoptosis, cycle, and invasive ability of 95D cells.

Results After 48 h of transfection, the level of miR-125b was increased significantly in the mimics transfection group, which was 22.32 times of the negative control group. The apoptosis rate of 95D cells in the miR-125b mimics transfection group(9.90±1.33)% was significantly lower than that in the negative control group(13.77±0.52)% (P < 0.05), but no significant changes were found in cell proliferation, cycle, and invasive ability (P>0.05).

Conclusion Over-expression of miR-125b could inhibit the apoptosis of lung cancer 95D cells.