Objective To investigate the effects of benzoapyrene (BaP) on the expression of connexin 43(CX43) and cell proliferation in mouse sertoli cells without change of cell apoptosis.
Methods The mouse sertoli cell line TM4 cells were exposed to varied concentrations of BaP (dimethyl sulfoxide control group,0.5 and 10.0 μmol/L BaP groups) for 4 h or 72 h.Then these cells were collected to measure cell viability,proliferation,and apoptosis.Besides,mRNA and protein expression levels of CX43 were also measured.
Results Compared with the control group,no difference in the viability,proliferation,apoptosis,or protein expression levels of CX43 was found in the TM4 cells with 4 h-exposure to 0.5 and 10.0 μmol/L BaP (P > 0.05),but the mRNA expression levels of CX43 was significantly increased (P < 0.05).For 72 h exposure,the viability and proliferation were significantly decreased (P < 0.05) in the TM4 cells without change of apoptosis,and the mRNA and protein expression levels of CX43 were significantly increased (P < 0.05).
Conclusion BaP exposure at 0.5 and 10.0 μmol/L could inhibit proliferation of mouse sertoli cells and increase mRNA and protein expression levels of CX43.
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