Objective To examine the effects on serum specific IgG antibody level in mice exposed to 2,4-toluene diisocyanate (2,4-TDI) at different doses and for different time.
Methods Specific pathogen free (SPF) BALB/c mice (female,n=64) were randomly divided by the exposure time of 7 or 14 days into two parts with a total of eight groups containing eight rats per group.Each part included control group,low (0.036 mg/m3),middle (0.14 mg/m3),and high (0.36 mg/m3) dose groups.Rats in the latter three groups were continuously exposed to 2,4-TDI vapor for 4 h/d.Weight was recorded and serum samples were collected 24 h following the final exposure.The levels of serum specific IgG were detected by enzyme-linked immunosorbent assay.
Results The average optical density level of specific IgG antibody in each dose group on day 7(0.14& #177;0.008 4,0.14& #177;0.006 4,0.14& #177;0.007 6) had no significant difference compared with the control group (0.14& #177;0.009 3)(P < 0.05).And the levels of specific IgG antibody on day 14 were different among the groups (F=11.41,P < 0.05).The results of pair-wise comparison showed that the middle dose group (0.17& #177;0.020 0) and the high dose group (0.18& #177;0.021 0) presented higher levels of specific IgG antibody than the control group (0.14& #177;0.004 6)(P < 0.05),and the high dose group also presented higher level of specific IgG antibody than the low dose group (0.15& #177;0.012 0)(P < 0.05).The middle dose group on day 14(0.17& #177;0.020 0) showed a higher level of specific IgG antibody than on day 7(0.14& #177;0.006 4)(P < 0.05).Similarly,the high dose group showed higher level of specific IgG antibody on day 14(0.18& #177;0.021 0) than on day 7(0.14& #177;0.007 6)(P < 0.05).
Conclusion Both concentration and time of TDI continuous exposure could influence the expression levels of serum specific IgG antibody.
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