CHEN Zhang-jian, HAN Shuo, ZHENG Pai, ZHOU Shu-pei, JIA Guang. Effect of subchronic combined oral exposure to titanium dioxide nanoparticles and glucose on blood coagulation in young rats[J]. Journal of Environmental and Occupational Medicine, 2020, 37(5): 503-507, 519. DOI: 10.13213/j.cnki.jeom.2020.19862
Citation: CHEN Zhang-jian, HAN Shuo, ZHENG Pai, ZHOU Shu-pei, JIA Guang. Effect of subchronic combined oral exposure to titanium dioxide nanoparticles and glucose on blood coagulation in young rats[J]. Journal of Environmental and Occupational Medicine, 2020, 37(5): 503-507, 519. DOI: 10.13213/j.cnki.jeom.2020.19862

Effect of subchronic combined oral exposure to titanium dioxide nanoparticles and glucose on blood coagulation in young rats

  • Background Animal experiments have shown that oral exposure to titanium dioxide (TiO2) nanoparticles can affect hematologic system. TiO2 nanoparticles are often found in high sugar food as food additives, and children are highly exposed to them because they have a sweet tooth.
    Objective This study aims to explore the effect of combined oral exposure to TiO2 nanoparticles and glucose on blood coagulation in young rats.
    Methods Eighty three-week-old clean SD rats were randomly divided into eight groups (10 rats in each group, half male and half female) after one week of adaptive feeding. By a 4×2 factorial design, the rats were exposed to TiO2 nanoparticles through intragastric administration at 0, 2, 10, and 50 mg·kg-1 (body weight) with or without 1.8 g·kg-1 (body weight) glucose daily for 90 days. During the experiment, the body weight of the rats was monitored once a week. After 90 days of exposure, blood coagulation indices such as activated partial thromboplastin time (APTT), prothrombin time (PT), and fibrinogen (FIB) were detected. Response surface model and general linear model were used for interaction analysis.
    Results Exposure to TiO2 nanoparticles and glucose did not change body weight of the rats. Only APTT in the female rats exposed to 10 mg·kg-1 TiO2 nanoparticles(33.90±2.05) s was higher than that in the control group(25.88±3.59) s (P < 0.05). The APTT(44.10±18.12) s vs (25.88±3.59) s and PT(26.40±4.21) s vs (21.38±1.04) s were increased in the female rats exposed to 50mg·kg-1 TiO2 nanoparticles and glucose compared with the control group, and PT(22.64±1.53) s and (21.68±2.19)s vs (24.96±1.05)s was decreased in the male rats exposed to 2mg·kg-1 and 10mg·kg-1 TiO2 nanoparticles and glucose and FIB(1.47±0.07)g·L-1 vs (1.76±0.08)g·L-1 was decreased in the male rats exposed to 50 mg·kg-1 TiO2 nanoparticles and glucose compared with the control group (P < 0.05). The interaction analysis results showed that TiO2 nanoparticles and glucose had a significant antagonistic effect on APTT and FIB in male rats (P < 0.05).
    Conclusion Subchronic combined oral exposure to TiO2 nanoparticles and glucose have an obvious antagonistic effect on blood coagulation in young SD rats. The combined effect of oral exposure to TiO2 nanoparticles and glucose is more obvious than that of TiO2 nanoparticles alone. In the case of real consumer exposure, the combined exposure effect of TiO2 nanoparticles and sugar needs concern.
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