ZHANG Hong, XU Feng-lan, ZHOU Rong, HU Xiao-ya, LUO Bing-zhi, ZHANG Zhe, LIU Xiao-qin, GUI Fang, SI Jing. Effects of combined exposure to lead and naphthalene on development of zebrafsh embryos[J]. Journal of Environmental and Occupational Medicine, 2018, 35(2): 95-101. DOI: 10.13213/j.cnki.jeom.2018.17550
Citation: ZHANG Hong, XU Feng-lan, ZHOU Rong, HU Xiao-ya, LUO Bing-zhi, ZHANG Zhe, LIU Xiao-qin, GUI Fang, SI Jing. Effects of combined exposure to lead and naphthalene on development of zebrafsh embryos[J]. Journal of Environmental and Occupational Medicine, 2018, 35(2): 95-101. DOI: 10.13213/j.cnki.jeom.2018.17550

Effects of combined exposure to lead and naphthalene on development of zebrafsh embryos

  • Objective To study the effects of individual and combined exposure to lead and naphthalene on the development of zebrafish embryos.

    Methods Zebrafish embryos (n=500) were randomly divided into 10 groups. Lead (0, 12, and 48 μmol/L) and naphthalene (0, 5, and 20 μmol/L) were administered individually or combined to zebrafish embryos from 8 h post fertilization until 144 h post fertilization. Mortality, hatchability, and malformation were observed, recorded, and calculated. Spontaneous movement, heart beats, and swimming speeds in response to photoperiod stimulation in larval zebrafish were recorded and analyzed.

    Results Compared to the control groups, exposure to 48 μmol/L lead or 20 μmol/L naphthalene caused a significant increase in mortality and a significant decrease in hatchability (P < 0.05), and exposure to 20 μmol/L naphthalene caused a significant in crease in malformation (P < 0.05). Compared to individual exposure at the same concentration:combined exposure to 12 μmol/L le ad+5 μmol/L naphthalene or 48μmol/L lead+20μmol/L naphthalene caused a significant increase in mortality; combined exposure to 48 μmol/L lead+20 μmol/L naphthalene caused a significant decrease in hatchability (P < 0.05); combined exposure to 12 μmol/L le ad+20 μmol/L naphthalene, 48 μmol/L lead+5 μmol/L naphthalene, or 48 μmol/L lead+20 μmol/L naphthalene caused a significant in crease in malformation (P < 0.05). There were interactions between lead and naphthalene on mortality (F=2.863, P < 0.05), hatchability (F=4.474, P < 0.05), and malformation (F=5.084, P < 0.05). Compared to the blank control group, the larval zebrafish's swimming speed was decreased after individual exposure to 48 μmol/L lead at the first dark period and the second and third light periods (P < 0.05). Compared to the acetone control group, the swimming speed was decreased after individual exposure to 20μmol/L naphthalene at all periods (P < 0.05). Compared to individual exposure at the same concentration, the larval zebrafish's swimming speed was decreased after combined exposure to 48μmol/L lead+20μmol/L naphthalene at the first dark period (P < 0.05). There was an interaction between lead and naphthalene on swimming speed (F=4.493, P < 0.05).

    Conclusion Exposure to lead and naphthalene could impair the development of zebrafish embryos and cause developmental neurotoxicity. Lead and naphthalene have interaction effects on the mortality, hatchability, and malformation of zebrafish embryos and neurobehavior of zebrafish larvae.

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