Objective To investigate the effects of phosgene exposure on hemorheological in dicators of rats.
Methods Health adult male SD rats were randomized into a control group (n=30) and a phosgene exposure group (n=30). Blood samples of rats were collected at 1, 3, 6, 12, and 24 h after 5-min air or phosgene (8.33 mg/L) inhalation and after intraperitoneal in jection with 20% urethane anesthesia. Routine blood test, blood gas analysis, and hemorheological indicator detection were performed.
Results The levels of arterial oxygen partial pressure and oxygenation index decreased at 3 h after phosgene inhalation and reached bottom at 6 h, being 58.67 and 202.30 mmHg (1 mmHg=0.133 kPa), respectively. The arterial oxygen partial pressure at 6 h reached the clinical standard of respiratory failure ( < 60 mmHg); the oxygenation index at 6 h reached the diagnostic criteria for acute lu ng injury ( < 300 mmHg) and was close to the diagnostic criteria for acute respiratory distress syndrome ( < 200 mmHg). Compared with the control group, the exposure group showed elevated partial pressure of carbon dioxide at each designed time point, and the trend of pH was aligned with oxygen partial pressure and oxygenation index. The red blood cell counts at 1, 3, and 24h were lower in the exposure group than in the control group (P < 0.01 or P < 0.05), except 6 and 12 h. Packed cell volume was increased in the exposure group and showed statistical differences at 6 and 12 h compared with the control group (P < 0.01). Platelet count was decreased in the exposure group at all time points (P < 0.05). In addition, the rats administered with phosgene showed higher whole blood viscosity than the control group at varied shear rates, especially at 6 h, and except the shear rate of 10 s-1, the whole blood viscosity at other shear rates showed statistical differences between the two groups (P < 0.01). The plasma viscosity of the exposure group was increased at 1h, then decreased, and no difference was found at 24h compared with the control group. The maximum erythrocyte deformability index of the exposure group was obviously decreased from 1h, reached bottom at 12, but rebounded slightly at 24h.
Conclusion Phosgene can induce acute lung injury and significant changes in hemorheological indicators in rats.
DownLoad: