LIU Xiao-li, LIU Fang-ying, MENG Chao, LI Ping, ZHANG Dian-ping, SONG Jing, LIU Ke-jian. Effects of fluoride exposure on expression of SOST and DKK1 in rat skin fbroblasts[J]. Journal of Environmental and Occupational Medicine, 2017, 34(10): 914-917. DOI: 10.13213/j.cnki.jeom.2017.17156
Citation: LIU Xiao-li, LIU Fang-ying, MENG Chao, LI Ping, ZHANG Dian-ping, SONG Jing, LIU Ke-jian. Effects of fluoride exposure on expression of SOST and DKK1 in rat skin fbroblasts[J]. Journal of Environmental and Occupational Medicine, 2017, 34(10): 914-917. DOI: 10.13213/j.cnki.jeom.2017.17156

Effects of fluoride exposure on expression of SOST and DKK1 in rat skin fbroblasts

  • Objective To observe the changes of protein expressions of sclerostin (SOST) and dickkopf-1 (DKK1) in fibroblasts of rat skin following fluoride exposure.

    Methods Rat skin fibroblasts were cultured with fluoride at different 0, 0.0001, 0.001, 0.01, 0.1, 1, 10, and 20mg/L. The expression le vels of SOST and DKK1 in cell culture supernatants were detected at four exposure time points (24, 48, 72, and 96h) by ELISA.

    Results The expression levels of SOST of the 0.000 1, 0.001, 0.01 mg/L fluoride groups at 48 h, the 1 and 10 mg/L fluoride groups at 72 h, and the 0.001, 0.01, 0.1, and 1 mg/L fluoride groups at 96 h were significantly lower than that of the 0 mg/L fluoride group (P < 0.05). The expression levels of SOST of the 0.01mg/L fluoride group at 48h and the 0.001, 0.01, 0.1, 1, and 10mg/L fluoride groups at 96 h were significantly lower than those of the groups at 24 h (P < 0.05). The expression levels of DKK1 of the 0.001 mg/L fluoride group at 48 h, the 0.01, 0.1, 1, 10, and 20 mg/L fluoride groups at 72 h, and the 0.01, 0.1, 1, 10, and 20 mg/L fluoride groups at 96h were significantly lower than that of the 0mg/L fluoride group (P < 0.05). The expression levels of DKK1 of the 0.0001, 0.001, 0.01 mg/L fluoride groups at 48 h, the 0.001, 0.01, 1, 10, and 20 mg/L fluoride groups at 72 h, and the 0.01, 0.1, 1, and 20 mg/L groups at 96 h were significantly lower than those of the groups at 24 h (P < 0.05).

    Conclusion Fluoride can cause down-regulation of expression of SOST and DKK1 in rat skin fibroblasts.

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