CHEN Qing, ZHAO Zong-xia, ZHAO Jin-yan, LI Xiang-hong, LU Xiao-ning, XUE Xiang. Effects of paraquat on semen quality and lipid peroxidation in testis of male rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(4): 337-340. DOI: 10.13213/j.cnki.jeom.2017.16656
Citation: CHEN Qing, ZHAO Zong-xia, ZHAO Jin-yan, LI Xiang-hong, LU Xiao-ning, XUE Xiang. Effects of paraquat on semen quality and lipid peroxidation in testis of male rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(4): 337-340. DOI: 10.13213/j.cnki.jeom.2017.16656

Effects of paraquat on semen quality and lipid peroxidation in testis of male rats

  • Objective To study the effects of paraquat (PQ) on semen quality and lipid peroxidation in testis of male rats.

    Methods Healthy adult male Wistar rats (n=32) were randomly divided into four groups, including control group and paraquat (0.5, 2.0, and 8.0 mg/kg) groups, with eight rats in each group. Animals were exposed to designed treatments by oral gavage for eight weeks and then sacrificed. Testis and epididymis were isolated and weighed, and organ coefficient was calculated. Sperm count and motility plus testes histopathological changes using HE staining were determined. Additionally, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-Px) activity of testis tissues were detected.

    Results The sperm counts (48.37±3.76)×106, (41.56±3.13)×106, and (32.48±4.09)×106 and mobility (68.85±5.27)%, (66.65±2.83), and (52.30±1.99)% of the 0.5, 2.0, and 8.0 mg/kg paraquat groups were less than those of the control group (53.19± 4.15)×106 and (70.65±6.07)% (P < 0.05) respectively. The organ coefficients of testis (0.87±0.08, 0.85±0.07, and 0.78±0.07) and epididymis (0.27±0.02, 0.29±0.03, and 0.31±0.03) of the paraquat groups were different from those of the control group (0.94±0.06 and 0.24±0.03) (P < 0.05) respectively. Obvious degenerative changes were observed in the paraquat treated rats. The 8.0 mg/kg paraquat exposure led to a significant increase in the MDA content (1.45±0.18) nmol/mg vs.(0.79±0.11) nmol/mg, and decrease in the SOD (76.19±6.71) μ/mg vs.(108.50±11.24) μ/mg and GSH-Px (15.07±2.71) μ/mg vs.(30.61±3.23) μ/mg activities of testes tissues.

    Conclusion Paraquat can result in decreased semen quality of rats and elevated lipid peroxidation in testis tissues.

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