ZHANG Rui, LIAO Qiu-xia, KE Lu-lu, ZHANG Si-meng, OUYANG Wei, ZHANG Zi-gui. Effects of calcium on mitochondrial injury of kidney in offspring rats with maternal fluoride exposure[J]. Journal of Environmental and Occupational Medicine, 2017, 34(2): 154-159. DOI: 10.13213/j.cnki.jeom.2017.16450
Citation: ZHANG Rui, LIAO Qiu-xia, KE Lu-lu, ZHANG Si-meng, OUYANG Wei, ZHANG Zi-gui. Effects of calcium on mitochondrial injury of kidney in offspring rats with maternal fluoride exposure[J]. Journal of Environmental and Occupational Medicine, 2017, 34(2): 154-159. DOI: 10.13213/j.cnki.jeom.2017.16450

Effects of calcium on mitochondrial injury of kidney in offspring rats with maternal fluoride exposure

  • Objective To study the effects of calcium on mitochondrial injury of kidney in offspring rats following maternal fluoride treatment via water drinking.

    Methods A total of 100 early weaning SD female rats were randomly divided into control group, fluoride group (100 mg/L NaF), low calcium group (0.063% CaCO3), low calcium plus fluoride group (100 mg/L NaF+0.063% CaCO3), and high calcium plus fluoride group (100 mg/L NaF+7% CaCO3). After three months of feeding, the rats were mated for 1:1 and the 14-and 28-day-old offspring rats were sampled to determine succinate dehydrogenase (SDHase) activity, malondialdehyde (MDA) level, renal cell apoptosis, and expression levels of fission/fusion proteins Fis1, Drp1, and Mfn2 in mitochondria.

    Results Compared with the fluoride group, the activity of SDHase was increased in the high calcium plus fluoride group (P < 0.05), and was decreased in the low calcium plus fluoride group (P < 0.01). Compared with the control group, the mitochondria MDA level and the apoptosis cells of the fluoride treated groups were significantly increased. Compared with the fluoride group, the apoptosis cells were increased in the low calcium plus fluoride group and decreased in the high calcium plus fluoride group. Compared with the control group, the expression levels of fission protein Fis1 were increased in the 14-day-old male offspring rats of the low calcium plus fluoride group (P < 0.05); the expression levels of fission protein Drp1 were increased in the 28-day-old male offspring rats of the low calcium plus fluoride group and the high calcium plus fluoride group (P < 0.05).

    Conclusion The results suggest that kidney mitochondrial impairment is caused by fluorosis through regulating the expression of fission/fusion proteins Fis1, Drp1, and Mfn2. High calcium intake can decrease lipid peroxidation in mitochondria, thus reducing fluorosis induced cytotoxicity to offspring rat's renal cells, but low calcium intake can exacerbate the toxic effect.

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