WANG Yu, XUE Hui-zhong, GAO Ming, TAN Yu-feng, SONG Yang, YANG Ye, FAN Hong-liang, ZHOU Cheng, WU Nan-xiang. Study of DNA Damage and Oxidative Stress of Human B Lymphocytes Induced by Methyl Tert-Butyl Ether[J]. Journal of Environmental and Occupational Medicine, 2016, 33(12): 1138-1142. DOI: 10.13213/j.cnki.jeom.2016.16291
Citation: WANG Yu, XUE Hui-zhong, GAO Ming, TAN Yu-feng, SONG Yang, YANG Ye, FAN Hong-liang, ZHOU Cheng, WU Nan-xiang. Study of DNA Damage and Oxidative Stress of Human B Lymphocytes Induced by Methyl Tert-Butyl Ether[J]. Journal of Environmental and Occupational Medicine, 2016, 33(12): 1138-1142. DOI: 10.13213/j.cnki.jeom.2016.16291

Study of DNA Damage and Oxidative Stress of Human B Lymphocytes Induced by Methyl Tert-Butyl Ether

  • Objective To examine the DNA damage and oxidative stress in human B lymphocytes exposed to different concentrations of methyl tert-butyl ether (MTBE).
    Methods Human B lymphocytes were exposed to MTBE at concentrations of 1.25,2.5,5,10,12.5 μmol/L for 24 h,separately.Then the treated cells were harvested to test percentage of tail DNA and Olive tail moment by using the comet assay.Meanwhile,the levels of malondialdehyde (MDA),glutathione peroxidase (GSH-Px),and 8-hydroxydeoxyguanosine (8-OHdG) in cell supernatant were detected by ELISA.Additionally,a correlation analysis was carried out between the concentrations of MTBE and the selected indicators.
    Results After the cells were treated for 24 h,the percentage of tail DNA and the olive tail moment in the groups exposed to 5,10,and 12.5 μmol/L MTBE were significantly higher than those in the control group (all Ps<0.01).As for the oxidative stress,the 8-OHdG levels were elevated in the groups exposed to 1.25,2.5,5,and 12.5 μmol/L MTBE compared to the control group (P<0.05);however,except the 10 μmol/L MTBE group,the MDA and GSH-Px levels in the other exposed groups showed no significant change,compared with those in the control group.And there was a significant positive correlation between the concentrations of MTBE and percentage of tail DNA and the Olive tail moment (r=0.685,P=0.000;r=0.615,P=0.000).
    Conclusion The study findings indicate that a low concentration of MTBE could induce DNA damage of human B lymphocytes,and DNA damage could be used as a sensitive indicator of MTBE toxicity.
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