LU Qing-qing, ZHANG Juan, YIN Li-hong, PU Yue-pu. Inhibitory Effect of Prodigiosin on Growth and Toxin-Producing Capability of Microcystis aeruginosa[J]. Journal of Environmental and Occupational Medicine, 2016, 33(2): 97-102. DOI: 10.13213/j.cnki.jeom.2016.15508
Citation: LU Qing-qing, ZHANG Juan, YIN Li-hong, PU Yue-pu. Inhibitory Effect of Prodigiosin on Growth and Toxin-Producing Capability of Microcystis aeruginosa[J]. Journal of Environmental and Occupational Medicine, 2016, 33(2): 97-102. DOI: 10.13213/j.cnki.jeom.2016.15508

Inhibitory Effect of Prodigiosin on Growth and Toxin-Producing Capability of Microcystis aeruginosa

  • Objective To explore the inhibitory effects of prodigiosin on the growth of Microcystis aeruginosa (M. aeruginosa) and the production of microcystins during different inhibition processes.

    Methods M. aeruginosa was cultivated to logarithmic phase, and then the effects of prodigiosin on growth were examined by cell counting and flow cytometry assay after exposure to six doses of prodigiosin between 0.156 and 5.000 μg/mL. The contents of reactive oxygen species(ROS), the activities of superoxide dismutase(SOD), the contents of DNA, and the intracellular and extracellular microcystins of M. aeruginosa were detected respectively after 24 h and 48 h exposure to 1.25, 2.50, and 5.00 μg/mL prodigiosin.

    Results The half maximal effective concentration(EC50) value of prodigiosin for growth inhibition of M. aeruginosa was 2.76 μg/mL(95% confidence interval, 0.96-7.91 μg/mL). The content of ROS was 8.47 and 19.60 respectively, and the activity of SOD was 14.24 and 5.31 U/mL respectively after 24 h exposure to 2.50 and 5.00 μg/mL prodigiosin; both were decreased compared to the control group. Damaged membrane integrity was observed in varying degrees after exposure to different doses of prodigiosin. The cell diameter of M. aeruginosa was 2.72, 2.73, and 2.75 μm respectively and was larger than that of normal cells(2.54 μm) after 24 h exposure to 1.25, 2.50, and 5.00 μg/mL prodigiosin; the average DNA content also elevated as 1.08, 1.18, 1.32 times of normal cells. Furthermore, the microcystin-LR production of M. aeruginosa was inhibited after exposure to prodigiosin; the most remarkable inhibitory effect was shown when 2.50 μg/mL prodigiosin was added and the content of microcystin-LR was reduced by 23.80% after 72 h.

    Conclusion Prodigiosin could induce cell oxidative damage and proliferation inhibition to inhibit the growth of M. aeruginosa. In addition, prodigiosin could reduce the production of microcystin-LR.

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