硝酸-过氧化氢脱蛋白-石墨炉原子吸收法测定全血中铅
Determination of Lead in Whole Blood by Graphite Furnace Atomic Absorbance Spectrometry with Nitric Acid-Hydrogen Peroxide Preparation for Protein Removal
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摘要:
目的 建立一种新的血铅石墨炉原子吸收快速测定法。 方法 采用含40 mL/L 硝酸与6 mL/L 过氧化氢的混合提取液,离心分离脱去全血中的蛋白,以磷酸二氢铵+硝酸镁为基体改进剂,石墨炉原子吸收光谱法直接上机测定。 结果 该方法的线性范围为10~400 μg/L,最低检出限为4 μg/L,加标回收率为87.8%~115.3%,相对标准偏差(RSD)为3.6%~8.6%。 结论 该新的血铅石墨炉原子吸收快速测定方法已成功应用于全血样品的铅测定,结果令人满意。 Abstract:Objective To establish a novel method for lead determination in whole blood by graphite furnace atomic absorbance spectrometry (GFAAS). Methods After the protein in whole blood was removed by 40 mL/L nitric acid mixed with 6 mL/L hydrogen peroxide in a centrifugal separator, lead in whole blood was determined by GFAAS with NH4H2PO4+Mg(NO3)2 as matrix modifier． Results Of the current methodological study, the linear range was 10-400 μg/L, the detection limit was 4 μg/L, the recovery rate range was 87.8%-115.3%, and the relative standard deviation (RSD) was 3.6%-8.6%． Conclusion Graphite furnace atomic absorbance spectrometry with nitric acid-hydrogen peroxide deproteinization is satisfied to the purpose of lead determination of whole blood.
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