孕期十溴联苯醚暴露对子代大鼠空间学习记忆能力的影响
Effects of Prenatal Exposure to Decabromodiphenyl Ether on Learning and Memory in Rat Offspring
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摘要:
目的 观察孕期十溴联苯醚(Decabromodiphenyl ether,BDE-209)暴露对子代大鼠活动能力及空间学习记忆能力的影响。 方法 将SD孕鼠随机分为对照组和100、300、900 mg/kg BDE-209 染毒组。于妊娠第6~20 天期间,3 个染毒组分别每天灌胃BDE-209 染毒,对照组采用玉米油灌胃,连续15 d。采用旷场实验观察子代大鼠活动能力,采用Morris 水迷宫实验检测大鼠空间学习及记忆能力。 结果 (1)旷场实验表明:各组子代大鼠在旷场中活动的总距离差异无统计学意义(雌性子代大鼠,F=2.13,P>0.05;雄性子代大鼠,F=0.70,P>0.05)。(2)Morris 水迷宫实验表明:在隐蔽平台实验中,训练第3 天,雌性子代大鼠的300 mg/kg 和900 mg/kg 染毒组与对照组相比,逃避潜伏期延长,差异具有统计学意义(P< 0.01);而雄性子代大鼠各组差异无统计学意义(P>0.05)。在空间探索实验中,雄性子代大鼠各组间平台象限活动时间的差异有统计学意义(F=4.17,P< 0.01),其中100 mg/kg 和300 mg/kg 染毒组与对照组相比,平台象限活动时间缩短,其差异有统计学意义(P< 0.01);而雌性子代大鼠各组间差异无统计学意义(P>0.05)。 结论 孕期BDE-209 暴露对子代大鼠活动能力无明显影响,可能导致雌性子代大鼠空间学习记忆能力下降和雄性子代大鼠空间位置记忆能力下降。 Abstract:Objective To investigate the effects of prenatal exposure to decabromodiphenyl ether (BDE-209) on learning and memory as well as locomotor activity in rat offspring. Methods Pregnant SD rats were randomly treated with BDE-209 (100, 300, and 900 mg/kg) or corn oil on gestational days 6-20. Behavioral performances of all the groups were evaluated by open field test and Morris water maze test. Results 1) In the open field test, no significant difference in travelled distance was found among all the groups (female offspring, F=2.13, P>0.05; male offspring, F=0.70, P>0.05). 2) In the hidden platform trials of Morris water maze experiment, the latency periods were significantly prolonged in the female offspring prenatally administered with 300 mg/kg and 900 mg/kg BDE-209 compared to the controls (P< 0.01), but no significant difference was observed in the male offspring (P>0.05). The results of probe trails showed that the male offspring presented statistically significant changes in the time spent in targeted quadrant (F=4.17, P< 0.01); compared to the controls, the 100 mg/kg and the 300 mg/kg male offspring groups showed significantly decreased time spent in targeted quadrant, but no significant difference was observed among the female offspring groups (P>0.05). Conclusion Prenatal exposure to decabromodiphenyl ether may cause spatial learning and memory impairments in female rat offspring, and no changes in locomotor activity was observed in both genders.
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