马萍, 陈姣娥, 甘亚平, 曾强, 毛琳, 杨旭. 氯氰菊酯对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用[J]. 环境与职业医学, 2013, 30(6): 465-470.
引用本文: 马萍, 陈姣娥, 甘亚平, 曾强, 毛琳, 杨旭. 氯氰菊酯对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用[J]. 环境与职业医学, 2013, 30(6): 465-470.
MA Ping , CHEN Jiao-e , GAN Ya-ping , ZENG Qiang , MAO Lin , YANG Xu . Oxidative Damages of Cypermethrin on Mouse Testis Tissues and Antioxidant Role of Vitamin E[J]. Journal of Environmental and Occupational Medicine, 2013, 30(6): 465-470.
Citation: MA Ping , CHEN Jiao-e , GAN Ya-ping , ZENG Qiang , MAO Lin , YANG Xu . Oxidative Damages of Cypermethrin on Mouse Testis Tissues and Antioxidant Role of Vitamin E[J]. Journal of Environmental and Occupational Medicine, 2013, 30(6): 465-470.

氯氰菊酯对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用

Oxidative Damages of Cypermethrin on Mouse Testis Tissues and Antioxidant Role of Vitamin E

  • 摘要: 目的 研究氯氰菊酯(CP)对小鼠睾丸组织的氧化损伤和维生素E 的抗氧化作用。

    方法 以36 只昆明小鼠为受试动物, 随机分为6 组, 包括1 个阴性对照组、3 个氯氰菊酯染毒组、1 个维生素E 组和1 个高剂量氯氰菊酯(40 mg/kg)加维生素E组(100 mg/kg), 染毒组按10、20 和40 mg/kg 3 个剂量水平, 维生素E的剂量为100 mg/kg, 小鼠灌胃染毒7 d。取睾丸称重测定脏器系数; 以睾丸组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量; 以睾丸细胞测定DNA-蛋白质交联(DPC)系数; 并制作睾丸组织切片, 观察其病理损伤。

    结果 随着氯氰菊酯染毒剂量的升高, 睾丸的脏器系数、ROS、MDA含量和DPC系数逐渐上升, GSH含量逐渐降低, 各指标呈一定的剂量-效应关系。与对照组比较, 染毒剂量为20 mg/kg 时, 睾丸脏器系数(0.568& #177;0.027)、ROS 含量(586.2& #177;43.94)、GSH含量(18.15& #177;2.351)、DPC 系数(0.186& #177;0.017)和MDA含量(2.051& #177;0.137)差异有统计学意义(P < 0.05, P < 0.01), 睾丸部分生精小管生精细胞减少, 生精细胞脱落管腔; 染毒剂量为40 mg/kg 时, GSH含量(17.13& #177;1.203)、脏器系数(0.643& #177;0.028)、ROS 含量(898.8& #177;100.23)、MDA含量(2.299& #177;0.157)和DPC系数(0.229& #177;0.020)差异有统计学意义(P < 0.05, P < 0.01), 睾丸生精细胞明显减少, 部分生精细胞脱落坏死, 阻塞管腔。高剂量染毒加维生素E组与高剂量染毒组相比较, 睾丸的脏器系数、ROS、MDA含量和DPC 系数均有下降, GSH含量上升。ROS 含量(550.4& #177;102.3)、GSH含量(24.65& #177;2.892)和MDA含量(1.291& #177;0.289)差异有统计学意义(P < 0.05); 睾丸的脏器系数(0.507& #177;0.026)和DPC 系数(0.139& #177;0.017)差异有统计学意义(P < 0.01)。

    结论 较高剂量(20、40 mg/kg)的氯氰菊酯能造成小鼠睾丸的氧化损伤和病理损伤, 且可以被维生素E的抗氧化作用所拮抗。

     

    Abstract: Objective To investigate the oxidative damages of cypermethrin (CP) on mouse testis tissues and the antioxidant role of vitamin E.

    Methods Thirty-six Kuming mice were randomly divided into 6 groups and administered with designed agents by garage for 7 d. The groups included 1 negative control group, 3 CP groups (10, 20, and 40 mg/kg respectively), 1 protection group (40 mg/kg CP + 100 mg/kg vitamin E), and 1 vitamin E group (100 mg/kg). Afterwards, the mice were sacrificed and the testis organ coefficients were calculated. Reactive oxygen species (ROS), glutathione (GSH), and malondialdehyde (MDA) contents in the testis tissue homogenates were measured. Meanwhile, DNA-protein crosslink (DPC) coefficients were estimated from the testis cell suspension. The other testis tissues were prepared for sections and then stained for pathological observations under.

    Results The testis contents of ROS & MDA, the organ coefficients, and the DPC coefficients increased gradually in a dose-dependent manner, whereas the GSH contents decreased accordingly. Compared with the control group, the organ coefficient (0.568& #177;0.027), ROS content (586.2& #177;43.94), GSH content (18.15& #177;2.351), DPC coefficient (0.186& #177;0.017), and MDA content (2.051& #177;0.137) in the 20 mg/kg CP group were significantly elevated (P < 0.05 or P < 0.01). Besides, the tissue section of the mice in the 20 mg/kg CP group indicated a reduction and shedding of spermatogenic cells in the seminiferous tubules. In the 40 mg/kg CP group, the GSH content (17.13& #177;1.203), organ coefficient (0.643& #177;0.028), ROS contents (898.8& #177;100.23), the MDA content (2.299& #177;0.157), and DPC coefficient (0.229& #177;0.020) were significantly higher than those of the control group (P < 0.01 or P < 0.05). The spermatogenic cells reduced drastically and some fell off and blocked seminiferous tubules in the tissue section of the mice in the 40 mg/kg CP group. However, compared to the 40mg/kg CP group, the protection group showed significantly lower levels of organ coefficient (0.507& #177;0.026), ROS (550.4& #177;102.3), MDA (1.291& #177;0.289), and DPC (0.139& #177;0.017) (P < 0.05 or P < 0.01) levels, and higher GSH content (24.65& #177;2.892) (P < 0.05).

    Conclusion CP at relatively higher doses (20 mg/kg and 40 mg/kg) can induce oxidative stress and pathological damages in mouse testis tissues, while vitamin E can protect against the damages induced by CP.

     

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