窦婷婷, 常秀丽, 吕文, 周志俊. 百草枯对人胚胎神经干细胞分化过程中Wnt通路分子表达的影响[J]. 环境与职业医学, 2013, 30(6): 411-415.
引用本文: 窦婷婷, 常秀丽, 吕文, 周志俊. 百草枯对人胚胎神经干细胞分化过程中Wnt通路分子表达的影响[J]. 环境与职业医学, 2013, 30(6): 411-415.
DOU Ting-ting , CHANG Xiu-li , L Wen , ZHOU Zhi-jun . Effects of Paraquat on Expression of Wnt Signaling Molecules in Differentiation of Human Embryonic Neural Stem Cells[J]. Journal of Environmental and Occupational Medicine, 2013, 30(6): 411-415.
Citation: DOU Ting-ting , CHANG Xiu-li , L Wen , ZHOU Zhi-jun . Effects of Paraquat on Expression of Wnt Signaling Molecules in Differentiation of Human Embryonic Neural Stem Cells[J]. Journal of Environmental and Occupational Medicine, 2013, 30(6): 411-415.

百草枯对人胚胎神经干细胞分化过程中Wnt通路分子表达的影响

Effects of Paraquat on Expression of Wnt Signaling Molecules in Differentiation of Human Embryonic Neural Stem Cells

  • 摘要: 目的 通过对神经干细胞Wnt 信号通路中关键分子β-catenin/DVL2 基因表达的研究, 探讨百草枯对分化中的神经干细胞Wnt/β-catenin 信号通路的影响。

    方法 以人胚胎神经干细胞为研究对象, 观察体外诱导分化情况, 并采用四甲基偶氮唑盐(MTT)法测定不同浓度百草枯对细胞活力的影响, 选取未观察到细胞毒性的浓度为0.10、1.00、10.00 μmol/L 的百草枯染毒, 在分化2、4、8、12 d 利用实时荧光定量多链聚合反应技术检测Wnt 信号通路关键分子β-cateninDVL2 mRNA的表达情况。

    结果 百草枯在100.00 μmol/L 时可明显抑制细胞活力(P < 0.01)。百草枯在神经干细胞分化期2、8、12 d 明显下调β-catenin mRNA的表达, 除10.00 μmol/L 染毒组在分化期8 d 以外, 其余各剂量组与对照组相比, 差异具有统计学意义(P < 0.01)。百草枯在神经干细胞分化期2、12 d DVL2 mRNA的表达明显下调, 除0.10 μmol/L染毒组在分化期12 d 该基因mRNA表达升高外, 其余各剂量组与对照组相比差异均有统计学意义(P < 0.01); 在分化期4 dDVL2 mRNA的表达明显上升, 各剂量组与对照组相比差异具有统计学意义(P < 0.01)。

    结论 百草枯抑制Wnt 信号通路中关键分子β-cateninDVL2 基因mRNA的表达, 最终可影响神经干细胞的分化过程。

     

    Abstract: Objective To explore the effect of paraquet (PQ) on the gene expression of critical molecules, β-catenin and DVL2, in Wnt signaling pathway during differentiation process of neural stem cells.

    Methods Using the ReNcell CX cell model, the differentiation induced in vivo was observed. After treatment with various concentrations of PQ, cell viability was measured by tetrazolium (MTT) assay. After the cells exposed with non-cytotoxic concentrations of 0.00, 0.10, 1.00, and 10.00 μmol/L PQ, real-time reverse transcription-polymerase chain reaction was adopted to detect mRNA expressions of β-catenin and DVL2 on 2, 4, 8, and 12 d of differentiation.

    Results The cell viability was significantly inhibited after administered with 100.00 μmol/L of PQ (P < 0.01). PQ significantly down-regulated β-catenin mRNA expressions on 2, 8, and 12 d of differentiation (P < 0.01) though no obvious changes were observed between the 10.00 μmol/L group and the control group on 8 d of differentiation. In addition, PQ significantly down-regulated DVL2 mRNA expressions on 2 d and 12 d of differentiation compared to the control group (P < 0.01) though DVL2 mRNA expression increased at 0.10 μmol/L after 12 d of differentiation. But the DVL2 mRNA expressions significantly increased on 4 d of differentiation (P < 0.01).

    Conclusion PQ can suppress key factors β-catenin and DVL2 in Wnt signaling, which could affect differentiation of human neural stem cells.

     

/

返回文章
返回