固相萃取-高效液相色谱法检测水中多种拟除虫菊酯类农药
Determination of Five Pyrethroids in Water Using Solid Phase Extraction High Performance Liquid Chromatography
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摘要:
目的 研究同时测定水中甲氰菊酯、氯氟氰菊酯、溴氰菊酯、氰戊菊酯、氯菊酯5种拟除虫菊酯类农药污染物的固相萃取-高效液相色谱方法。 方法 水样经C18小柱富集,以异丙醇洗脱,采用光电二极管阵列检测器检测,利用C18色谱柱分离,以乙腈溶液和纯水为流动相等梯度洗脱,流速1.0 mL/min,在波长205 nm进行测定,外标法定量。 结果 5种拟除虫菊酯类农药在0.0~5.0 mg/L浓度范围内具有良好的线性关系,方法最低检测质量浓度为0.025~0.050 μg/L,回收率范围为88.0%~102.2%,相对标准偏差为1.0%~3.8%。 结论 该固相萃取-高效液相色谱法灵敏、准确,适用于水源水及生活饮用水中痕量拟除虫菊酯类农药残留的检测。 Abstract:Objective To establish a method for the simultaneous determination of 5 pyrethroids in source water or drinking water, i.e. fenpropathrin, cyhalothrin, deltamethrin, fenvalerate, and permethrin, using solid phase extraction combined with high performance liquid chromatography. Methods The water samples were extracted and condensed with solid-phase extraction using C18 cartridges and eluted by isopropanol. Separation was carried out on a Waters Sunfire C18 column, using acetonitrile and water as mobile phase gradient model at a flow rate of 1.0 mL/min, with detective wavelength at 205 nm. Quantification was calculated by an external standard method. Results The linear range of proposed determination methodology was 0.0-5.0 mg/L. The minimum detectable concentration was 0.025-0.050 μg/L. The recoveries were between 88.0% and 102.2% with a relative standard deviation in the range of 1.0%-3.8%. Conclusion The method is sensitive, accurate, and applicable to detect the trace pyrethroid residues in source water and drinking water.
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