CS2吸入染毒对雄性大鼠下丘脑-垂体-性腺轴超微结构的影响及NO干预作用
Ultrastructural Changes of Hypothalamus-Pituitary-Gonad Axis after Inhalation of Carbon Disulfide in Male Rats and The Intervention of Nitric Oxide
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摘要:
目的 探讨二硫化碳(CS2)对雄性大鼠下丘脑-垂体-性腺轴超微结构的影响及一氧化氮(NO)的干预作用。 方法 将24 只雄性SD 大鼠按体重随机分为4 组:对照组、CS2 染毒组、硝普钠(sodium nitroprusside,SNP)干预组和N-甲基-L-精氨酸(L-NMMA)干预组。除对照组外,其余3 组均以1 250 mg/m3 CS2 进行静式吸入染毒,2 h/d,5 d/ 周,共10 周,SNP 和L-NMMA干预组在动物染毒结束前10 d 开始分别腹腔注射SNP(5 mg/kg)和L-NMMA(2 mg/kg),1 次/d。染毒结束后,取下丘脑、垂体和睾丸组织,利用透射电子显微镜观察组织超微结构的改变。 结果 CS2 染毒可造成下丘脑神经元、垂体促性腺激素细胞、生长激素细胞和睾丸支持细胞线粒体肿胀,内质网扩张,SNP 对CS2 引起的下丘脑、垂体、睾丸组织的损伤具有拮抗作用,而L-NMMA则进一步导致病变的发生。 结论 CS2 可造成下丘脑、垂体和睾丸组织超微结构的改变,NO在此过程中发挥重要作用。 Abstract:Objective To study the ultrastructural changes of hypothalamic-pituitary-gonadal axis (HPGA) in carbon disulfide (CS2) treated rats and the interventional effect of nitric oxide (NO). Methods Twenty-four Sprague-Dawley male rats were randomly divided into 4 groups as: control, CS2, sodium nitroprusside (SNP) intervention and N-methyl-L-arginine (L-NMMA) intervention groups. Three experimental groups were treated with 1 250 mg/m3 CS2 by inhalation for 10 weeks, 2 h/d, 5 d/w. Two intervention groups received intraperitoneal injection of SNP (5 mg/kg), a nitric oxide donor, and L-NMMA (2 mg/kg), a nitric oxide synthase inhibitor, once a day for 10 days before the end of CS2 exposure. Then ultramicropathology technique was used to observe the ultrastructure of hypothalami, pituitaries and testes isolated from rats. Results CS2 induced swelling of hypothalamic neurons, gonadotropin cells, growth hormone cells and sertoli cells in male rats as well as widened endoplasmic reticulum. SNP reduced the effects of CS2 on ultrastructural changes; however L-NMMA enhanced the effects. Conclusion CS2 could induce ultrastructural changes of hypothalamus, pituitary and testis in male rats, which is mediated through NO.
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