多药耐药大肠杆菌对4种消毒剂的抗性研究
Resistance of Multidrug Resistant Escherichia Coli to Four Kinds of Disinfectants
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摘要:
目的 调查多药耐药大肠杆菌对临床常用消毒剂的抗性,为合理选择敏感、高效能的消毒剂提供参考依据。 方法 收集某医院临床分离出的多药耐药大肠杆菌,利用纸片琼脂扩散法和营养肉汤稀释法分别测定其对医院常用消毒剂的最低抑菌浓度(minimum inhibitory concentration,MIC),并用标准菌作为对照。 结果 不同消毒剂对多药耐药大肠杆菌的MIC值与产品推荐浓度存在差异,安尔碘Ⅱ型皮肤消毒剂(有效碘)为(17.5& #177;6.8) mg/L,洁芙柔消毒凝胶(DP300)为(2.9& #177;1.8) mg/L;3M爱护佳免洗手消毒液(葡萄糖酸氯己定)为(145.8& #177;85.4) mg/L,绿莎新爱尔施牌消毒片(有效氯)为(2 611.7& #177;1 083.0) mg/L。 结论 4种消毒剂对多药耐药大肠杆菌的实际使用浓度与推荐浓度存在差异,调整消毒剂使用浓度,对延缓细菌抗性的产生、控制医院感染有重要意义。 Abstract:Objective To test the resistance of multidrug resistant Escherichia coli to common hospital disinfectants, so as to provide reference for choosing sensitive and high efficiency disinfectants. Methods The multidrug resistant Escherichia coli were isolated and collected from clinical specimens. K-B disk diffusion method and nutrient broth dilution method were used to determine the minimum inhibitory concentrations (MIC) of four kinds of disinfectants. Standard strains were processed in parallel as the controls. Results The MIC of four disinfectants to the multidrug resistant Escherichia coli were different from their recommended concentrations. The MIC of An'erdian Skin Antiseptic II (available iodine) was (17.5& #177;6.8) mg/L; that of Jifro Hand Antiseptic Rinse Free Gel (DP300, triclosan) was (2.9& #177;1.8) mg/L; that of 3M Avagards Antiseptic Hand Rub (chlorhexidine gluconate) was (145.8& #177;85.4) mg/L; and that of L& #252;shaxin Aiershi Disinfectant Tablet (available chlorine) was (2 611.7& #177;1 083.0) mg/L. Conclusion There are differences between the actual concentrations of four disinfectants and their recommended concentrations. Corresponding adjustment to the concentrations can delay the occurrence of bacterial resistance and also has an important significance for the control of nosocomial infection.
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