X射线全身照射对大鼠血清氨基转移酶活性和肝脏结构的影响
Effects of Whole Body X-Ray Irradiation on Serum Transaminase Activity and Liver Structure in Rats
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摘要:
目的 观察不同剂量X射线全身照射对大鼠血清氨基转移酶活性及肝脏结构的影响。 方法 Wistar大鼠40 只,随机分为4 组:对照组、2 Gy 组、4 Gy 组、6 Gy 组。除对照组外,其余3 组大鼠按处理剂量行X射线全身单次照射。照射后第2 天,经腹主动脉取血、分离血清,测量谷氨酸氨基转移酶(alanine transaminase,ALT)、天冬氨酸氨基转移酶(aspartate transaminase,AST);取肝脏组织制作病理切片,观察肝脏的光学显微镜、电子显微镜结构。 结果 4 Gy、6 Gy 组ALT 水平升高,与对照组相比,差异具有统计学意义(P < 0.01);各照射组AST 值与对照组相比,差异无统计学意义(P > 0.05)。光镜结果显示,各照射组未见明显异常;电镜结果显示,6 Gy 组细胞器的改变多见于内质网,其结构松散,出现大量空泡。 结论 4 Gy、6 Gy X射线全身照射可对大鼠血清氨基转移酶活性造成影响,6 Gy X射线照射可对肝脏结构造成损害。 Abstract:Objective To study the effects of whole body X-ray irradiation on serum transaminase indices and structure of liver in rats. Methods Forty Wistar rats were randomly divided into four groups as blank control and other groups exposed to varied doses of X-ray (2 Gy, 4 Gy, and 6 Gy). Except those in the control group, the other 3 groups of rats were exposed to a single X-ray irradiation to the whole body with the designed doses. On the second day after irradiation, the serum alanine transaminase (ALT) and aspartate transaminase (AST) were assayed by sampling blood from abdominal aorta. The structure of liver was observed under optical microscope and electron microscope. Results Compared with the control group, the ALT levels of the 4 Gy and 6 Gy groups were significantly elevated (P < 0.01). There was no significant differences in the AST levels between the control group and the irradiation groups (P > 0.05). No apparent changes in hepatic structure were found under the optical microscope. However, vacuolated endocytoplasmic reticulum was identified in the 6 Gy group under electron microscope. Conclusion The serum transaminase indices of rats are changed after X-rays irradiation at 4 Gy and 6 Gy, and 6 Gy irradiation could damage hepatic structure.
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