温室土壤有机提取物遗传毒性研究
Genotoxicity of Extractable Organic Matter in Greenhouse Soil
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摘要:
目的 检测温室土壤中有机提取物的遗传毒性作用。 方法 以小鼠为实验动物,共分5 组:阴性对照组(二甲亚砜)、阳性对照组(环磷酰胺)及土壤有机提取物低、中、高剂量组,染毒剂量分别为5、15 和30 g 土壤干重/(kg 小鼠体重& #183;d),染毒方式为每日灌胃1 次,连续染毒4 周。用胞质分裂阻滞微核细胞试验和彗星试验分别检测有机提取物所致小鼠外周血淋巴细胞的染色体损伤作用和外周血细胞的DNA损伤作用。 结果 有机提取物剂量与微核率具有较明显的剂量- 效应关系,显著高于阴性对照组;随着有机提取物作用剂量的增加,彗星尾长、尾部DNA含量和Olive 尾矩显著升高。 结论 温室土壤中有机提取物可以诱使小鼠发生染色体和DNA损伤。 Abstract:Objective To study the genotoxicity of extractable organic matter (EOM) in vegetable greenhouse soil. Methods EOM obtained by soxhlet extraction from greenhouse soil were fed to 3 groups of mice via gavage at concentrations of 5, 15, and 30 g soil dry weight/kg body weight, once per day for 4 weeks. A negative control group was administered with dimethyl sulfoxide and a positive control group with cyclophosphamide. Chromosomal damage in peripheral blood lymphocytes and DNA injury in peripheral blood cells were detected by cytokinesis-block micronucleus (CBMN) assay and comet assay independently. Results There were significant differences in the micronucleus frequency between the EOM exposure groups and the negative control group, and the micronucleus frequency remarkably increased with EOM concentration. Compared with the negative control group, the tail length, tail DNA percent, and Olive tail moment increased in the middle and the high dose groups. Conclusion EOM extracted from greenhouse soil could induce chromosomal damage and DNA injury in mice.
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