几种螯合剂对锰致小鼠肺脏和睾丸毒性的解毒作用比较
Effects of Aroclor1254 on Embryo Development,Histology and Vitellogenin Expression in Liver of Zebrafish
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摘要:
目的 寻找理想的锰中毒解毒剂。 方法 小鼠一次性腹腔注射MnCl2(100 mg MnCl2/kg)24 h后分别给予L-组氨酸(L-His)、乙二胺四乙酸(EDTA)、二乙基二硫代氨基甲酸钠(DDTC)、meso-2, 3-二巯基丁二酸(DMSA)及N-乙酰-L-半胱氨酸(NAC)等五种螯合剂(2 mmol/kg,腹腔注射),观察小鼠肺脏、睾丸脂质过氧化物值(LPO)和组织中锰、钙、锌浓度的变化。 结果 小鼠一次性腹腔注射MnCl(2 100 mg MnCl2/kg)2 d后,小鼠肺脏和睾丸中LPO值分别为(2.42& #177;0.29)和(2.74& #177;0.33)μmol/g.prot,锰浓度分别为(2.60& #177;0.33)和(2.41& #177;0.68)μg/g,均明显高于对照组,差异有统计学意义(P<0.05)。分别染锰24 h后腹腔注射各螯合剂,治疗24 h后L-His对锰引起的肺脏、睾丸LPO值和锰浓度的升高有显著的抑制作用。 结论 L-His对锰致小鼠肺脏和睾丸毒性有较好的解毒作用,且自身毒性小,有望成为理想的锰解毒剂。 Abstract:Objective To develop new antidotes of manganese (Mn). Methods Mice were injected intraperitoneally with MnCl2(100 mg MnCl2/kg)24 h before they were injected intraperitoneally with chelating agents,including L-Histidine (L-His),ethylene diaminetetraacetic acid (EDTA),Diethyldithiocarbamate (DDTC),meso-2,3-dimercapto succinc acid (DMSA) and N-acetylcysteine (NAC).The effects of those chelating agents on lipid peroxidation (LPO) and concentrations of Mn,Ca,Zn in lung were observed. Results The Mn injection significantly increased the LPO levels (2.42& #177;0.29) and (2.74& #177;0.33)μmol/(g.prot) respectively as well as the concentrations of Mn (2.60& #177;0.33) and (2.41& #177;0.68)μg/g wet tissue respectively in lung and testes of mice (P<0.05) in 2 days.L-His effectively prevented the increases of above indices. Conclusion L-His is more effective than the other chelating agents in preventing changes caused by acute exposure to Mn.L-His is a promising antidote to Mn.
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