屈艾, 孙玲, 闫晶晶, 刘小燕, 沈益宏. 氯化镨对小鼠骨髓细胞微核率和核异常率的影响及硒的干预作用[J]. 环境与职业医学, 2011, 28(9): 547-550.
引用本文: 屈艾, 孙玲, 闫晶晶, 刘小燕, 沈益宏. 氯化镨对小鼠骨髓细胞微核率和核异常率的影响及硒的干预作用[J]. 环境与职业医学, 2011, 28(9): 547-550.
QU Ai , SUN Lin , YAN Jing-jing , LIU Xiao-yan , SHEN Yi-hong . Effects of Praseodymium Chloride on Rates of Bone Marrow Micronuclei and Nucleus Abnormity Rate in Mice and Selenium Intervention[J]. Journal of Environmental and Occupational Medicine, 2011, 28(9): 547-550.
Citation: QU Ai , SUN Lin , YAN Jing-jing , LIU Xiao-yan , SHEN Yi-hong . Effects of Praseodymium Chloride on Rates of Bone Marrow Micronuclei and Nucleus Abnormity Rate in Mice and Selenium Intervention[J]. Journal of Environmental and Occupational Medicine, 2011, 28(9): 547-550.

氯化镨对小鼠骨髓细胞微核率和核异常率的影响及硒的干预作用

Effects of Praseodymium Chloride on Rates of Bone Marrow Micronuclei and Nucleus Abnormity Rate in Mice and Selenium Intervention

  • 摘要: 目的 观察氯化镨(PrCl3)对小鼠骨髓细胞微核率和核异常率的影响,以及亚硒酸钠(Na2SeO3)的干预作用。

    方法 试验动物为5~6周龄昆明种小鼠;试验设置12个组,即5个氯化镨剂量组7.5、15、30、60、120 mg(/kg& #183;bw)、5个亚硒酸钠干预组在5个PrCl3剂量的基础上分别加入0.05 mg(/kg& #183;bw)的亚硒酸钠、2个对照组(阴性组为0.85%生理盐水,阳性组为叠氮化钠(NaN3), 20 mg(/kg& #183;bw)对照组。各组小鼠采用腹腔注射染毒或给药,运用微核测试技术和细胞核检测按照常规标准观察小鼠骨髓细胞的微核率(FMN)和核异常率(FAN),比较亚硒酸钠干预组对小鼠骨髓细胞FMN和FAN的影响。

    结果 在7.5~120 mg(/kg& #183;bw)的氯化镨剂量内,可致小鼠骨髓细胞FMN、FAN显著增高,并呈现剂量-效应关系(rFMN=0.885, rFAN=0.914);将亚硒酸钠干预组与氯化镨剂量组两两对应相比较,亚硒酸钠干预组的小鼠骨髓细胞FMN及FAN均有下降,经t检验显示,除7.5 mg(/kg& #183;bw)组差异无统计学意义以外,其余4组差异均有统计学意义(P<0.05或0.01)。

    结论 氯化镨提高了小鼠骨髓细胞核FMN及FAN,具有遗传损伤作用;加入亚硒酸钠能够降低小鼠细胞核的FMN及FAN,具有明显的干预作用。

     

    Abstract: Objective To investigate the effects of Praseodymium Chloride (PC) on rates of bone marrow micronuclei and nucleus abnormity in mice,as well as possible interventional effect of sodium selenite.

    Methods Five to six-week old Kunming mice of were divided into 12 groups:five exposure groups with doses at 7.5,15,30,60,120 mg/(kg& #183;bw) of PC,five treatment groups with the same five PC doses added 0.05 mg/(kg& #183;bw) Selenium respectively,negative (0.85% normal saline) and positive (sodium nitride,20 mg/(kg& #183;bw) groups.All doses were exposed via intraperitoneal injection and standard micronucleus test and nuclear test were followed.Mouse marrow cell frequency micronucleus (FMN) and frequency abnormal nuclei (FAN) were counted and the impacts of FMN and FAN after being added selenite sodium were observed.

    Results The 7.5-120 mg/(kg& #183;bw) doses of PC induced a remarkable increase of FMN and FAN and showed a significant correlation between the dosages and their effects (rFMN=0.885,rFAN=0.914).In the pairwise comparison of the treatment groups and the exposure groups,the FMN and FAN decreased after adding sodium selenite (t-test,P<0.05 except in the 7.5 mg/(kg& #183;bw) PC groups).

    Conclusion FMN and FAN of mouse marrow cell nucleus increase by exposed to PC,which indicates PC with possible genetic damage effects.Furthermore,sodium selenite could have intervention effects to PC exposure by reducing FMN and FAN.

     

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