黄芩苷对急性呼吸窘迫综合征大鼠心功能的影响
Effect of Baicalin on Rat Cardiac Function During Acute Respiratory Distress Syndrome
-
摘要:
目的 探讨黄芩苷对急性呼吸窘迫综合征(ARDS)大鼠心功能的影响。 方法 将120只健康成年雄性SD大鼠随机分为对照组、ARDS模型组和黄芩苷干预组,经股静脉注射油酸(0.12 mL/kg)复制ARDS模型,干预组同时腹腔注射黄芩苷溶液(300 mg/kg);在不同时间分别插管至颈动脉、右心室、左心室,记录血压、右心室收缩末期压力(RVSP)、左心室收缩末期压力(LVSP)、收缩末期左心室压力最大上升速率(+dp/dtmax)及舒张末期左心室压力最大下降速率(-dp/dtmax);之后采集动脉血测血氧分压。 结果 模型组大鼠动脉氧分压在注射油酸后10min时与对照组比较有明显下降(P<0.01),氧合指数2h时为(189.45& #177;43.03)mmHg (7.5mmHg=1kPa),达到了ARDS的诊断标准(<200mmHg);干预组氧分压和氧合指数在各时间点均低于对照组(P<0.01),在30 min及1、2、6、12、24 h均高于模型组(P<0.05或0.01)。模型组平均动脉压、RVSP、+dp/dtmax、LVSP等指标与对照组比较明显下降;干预组上述指标均较模型组明显改善。 结论 ARDS时心脏功能受到明显影响,尤以右心为著;黄芩苷可改善ARDS时的缺氧状态,也有助于改善心脏功能。 Abstract:Objective To investigate the effect of baicalin on rat cardiac functions during acute respiratory distress syndrome (ARDS). Methods A total of 120 rats were divided into three groups:the control group,the ARDS group and the baicalin-treated group.The rats of ARDS group were injected with 0.12 mg/kg of oleic acid through right femoral vein;while in the baicalin-treated group,300 mg/kg of baicalin were given through intraperitoneal injection,and equal volume of saline was administered by peritoneal injection in the control group.Then inserted heparinized catheters into left ventricles,right ventricles and carotid artery of the experimental rats,observe the changes of left ventricular end-systolic pressure (LVSP),+dp/dtmax,-dp/dtmax,right ventricular end-systolic pressure (RVSP),blood pressure by a polygraph and arterial blood gas analysis by a blood-gas Analyzer at 10 min,30 min,1 h,2 h,3 h,6 h,12 h and 24 h after injections,respectively. Results The level of arterial oxygen partial pressure decreased (P<0.01 vs.control) and met the diagnostic standard of ARDS at 2 hoxygenation index was (189.45& #177;43.03) mmHgin the ARDS group.In baicalin administration group,the level of arterial oxygen partial pressure and oxygenation index were higher than the ARDS group (P<0.01 or P<0.05).Compared with the ARDS group,all the other indices performed in the study were obviously improved in thebaicalin-treated group,such as LVSP,+dp/dtmax,-dp/dtmax,RVSP and blood pressure. Conclusion Cardiac dysfunction is observed in ARDS,especially right heart dysfunction;baicalin could improve anoxicity and cardiac function in ARDS.
下载:
