海尔福治疗慢性氟中毒小鼠的实验研究
Therapeutic Effect of Haierfu on Chronic Fluoride Poisoning in Mice
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摘要:
目的 研究海尔福对慢性氟中毒小鼠的治疗效果,为防治地方病氟中毒提供有效的药物。 方法 将30只健康小鼠随机分为3组,对照组、染毒组100 mg/L氟化钠(NaF)及治疗组(100 mg/L NaF+海尔福口服液)进行治疗。治疗后,通过测定血、股骨、粪便的氟含量,测定并分析肾组织上清液中脂质过氧化代谢产物:丙二醛(MDA)含量,超氧化物歧化酶(SOD)、谷光苷肽过氧化物酶(GSH-Px)的活性,以观察海尔福对氟中毒小鼠的治疗效果。 结论 血氟、骨氟含量:均以染毒组最高,分别是(5.68& #177;1.42) mol/L、(2.97& #177;1.92) mol/L;治疗组分别是(3.53& #177;0.68) mol/L、(1.19& #177;0.76) mol/L。染毒组与治疗组比较,差异有统计学意义(P<0.01);粪氟含量以治疗组最高(0.76& #177;0.18) mol/L,与其他2组比较差异有统计学意义(P<0.01);肾组织中MDA含量、GSH-Px和SOD活性;中毒组分别是(146.18& #177;17.23)μmol/L、(100.23& #177;16.34)& #215;103 U/L、(92.35& #177;7.83) U与对照组分别是(9.05& #177;2.62)μmol/L、(379.08& #177;10.02)& #215;103 U/L、(342.04& #177;13.65) U及治疗组分别是(72.46& #177;5.83)μmol/L、(340.52& #177;11.06)& #215;103 U/L、(261.87& #177;11.46) U比较,差异均有统计学意义(P<0.01)。 结论 海尔福可能通过促进氟的排泄,在一定程度上恢复SOD、GSH-Px的活性,对慢性氟中毒小鼠有一定的治疗作用。 Abstract:Objective To study the effects of Haierfu on mice treated with fluoride and provide evidences forendemic fluorosis treatment. Methods A total of 30 mice were divided into 3 groups randomly:the control group, the model group (100 mg/L Naf)and the treated group (100 mg/L Naf+ Haierfu oral solution). After the treatement applied, observed histological changes, determined fluoride in blood, bone tissue and feces, as well as malony diadehyde (MDA), superoxide dismutase (SOD) activity and glutathione peroxidase(GSH-Px) in kidney tissues. Results The fluorides in blood and bone tissue were the highest in the model group(5.68& #177;1.42) mol/L) and(2.97& #177;1.92) mol/L, respectively, compared to the treated group(3.53& #177;0.68)mol/L and (1.19& #177;0.76)mol/L), P<0.01. The fluoride in feces was higher in the treated group (0.76& #177;0.18)mol/L), compared to the other groups, P<0.01. The MDA, the activities of SOD and GSH-Px in the kidney tissue of the model group were (146.18& #177;17.23) μmol/L,(100.23& #177;16.34)& #215;103 U/L,(92.35& #177;7.83) U, respectively; and(9.05& #177;2.62) μmol/L,(379.08& #177;10.02)& #215;103 U/L and (342.04& #177;13.65)U in the control group respectively and (72.46& #177;5.83)μmol/L(, 340.52& #177;11.06)& #215;103 U/L, (261.87& #177;11.46)U in the treated group respectively.The model group showed higher values in these three items than the other two groups, P<0.01. Conclusion Haierfu could play some treating role to mice having fluorosis.
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