焦炉工人外周血淋巴细胞凋亡特征分析
Characteristics of Peripheral Blood Lymphocytes Apoptosis in Coke-oven Workers
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摘要:
目的 研究焦炉工人外周血淋巴细胞凋亡特征及其影响因素,为加强焦炉工的健康防护提供指导依据。 方法 收集343名焦炉工人班后尿,高效液相色谱法测定尿1-羟基芘(1-OHP)浓度,按尿1-OHP浓度将研究对象分为低、中、高暴露组。分光光度法测定血浆乳酸脱氢酶(LDH)活性和丙二醛(MDA)含量,流式细胞仪测定淋巴细胞凋亡率。 结论 以低暴露组淋巴细胞凋亡率95%上限值(7.90%)为界值判断淋巴细胞凋亡阳性,结果显示,低、中、高暴露组淋巴细胞凋亡阳性率随剂量升高而升高(趋势检验P<0.01)。经多因素Logistic回归分析,中暴露组、高暴露组和夜班是淋巴细胞凋亡阳性的主要危险因素(OR分别为3.42、8.58和5.05,P值均小于0.05)。相关分析结果显示,淋巴细胞凋亡率与尿1-OHP含量呈正相关(r=0.113,P<0.05)。 结论 焦炉逸散物暴露可导致部分焦炉工人淋巴细胞凋亡率升高,且与尿1-OHP水平有关。夜班是主要影响因素。 Abstract:Objective To investigate the characteristics of lymphocytes apoptosis in peripheral blood of coke-oven workers and its influential factors. Methods In this study, a total of 343 coke-oven workers were included. 1-Hydroxypyrene (1-OHP) in worker's post-shift urine sample was determined by high performance liquid chromatography. Subjects were divided into low, medium or high exposure group according to the 1-OHP concentration. Lactic dehydrogenase(LDH) activity and malondialchehyche (MDA)concentration in plasma were determined using spectrophotometry. Lymphocyte apoptosis in peripheral blood was determined using flow cytometry. Results The upper bound lymphocyte apoptosis rates of 95% subjects in the low exposure group (7.9%) was defined as the threshold to determine positive lymphocyte apoptosis. The positive rate of lymphocyte apoptosis in the low, medium and high exposure groups were increased with the exposure levels (chi-square trend test, P<0.01). The multivariate Logistic regression analysis showed that the medium exposure group, the high exposure group and the night work shift were the main risk factors of positive lymphocyte apoptosis (OR were 3.42, 8.58 and 5.05, P<0.05). The results of correlation analysis showed that lymphocyte apoptosis significantly correlated with urinary 1-OHP levels (r was 0.113, P<0.05). Conclusion Occupational exposure of coke-oven result in an increase possibility of lymphocyte apoptosis, which relate to urinary 1-OHP level. Night work shift is a predictive factor of lymphocyte apoptosis occurrence.
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