氯化汞致大鼠肾损伤及原花青素的保护作用
The Renal Damage in Rat Caused by Mercuric Chloride and the Protective Effect of Procyanidin
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摘要:
目的 观察氯化汞对大鼠肾脏的损伤作用及原花青素的保护作用。 方法 30只Wistar大鼠按体重随机分成5组,第1~4组以大豆油灌胃,第5组以原花青素450 mg/kg灌胃;2 h后,第1组皮下注射生理盐水,第2~5组分别皮下注射2.2、4.4、8.8、8.8 μmol/kg氯化汞。第2天重复上述步骤后,收集24 h尿液测定尿汞(Hg)和蛋白含量,以及碱性磷酸酶(ALP)、β-N-乙酰氨基葡萄糖苷酶(NAG)和乳酸脱氢酶(LDH)活力;腹主动脉采血测定血清尿素氮(BUN);切取肾皮质测定Hg含量和还原型谷胱甘肽(GSH)、丙二醛(MDA)水平及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力。 结论 与对照组比较,各染毒组的尿蛋白含量、尿LDH活力、肾皮质Hg含量、GSH、MDA含量及SOD、GSH-PX活力差异均具有统计学意义(P<0.05或P<0.01);4.4、8.8 μmol/kg染毒组的尿汞含量、ALP、NAG活力、血清BUN含量的差异均具有统计学意义(P<0.01)。原花青素干预组与8.8μmol/kg染毒组比较,尿LDH、ALP、NAG活力降低,尿蛋白含量下降,肾皮质GSH、MDA含量下降,SOD、GSH-Px活力升高,血清BUN含量下降,差异均具有统计学意义(P<0.05或P<0.01)。 结论 氯化汞可蓄积于大鼠肾脏并产生损伤,原花青素对大鼠氯化汞所致肾损伤具有一定程度的保护作用。 Abstract:Objective To observe the renal damage induced by mercuric chloride, and to investigate the protection effect of procyanidin on the renal damage induced by mercuric chloride. Methods Thirty Wistar rats were randomly divided into five groups by weight. Soya bean oil was given to the rats in first, second, third and fourth groups via gavage, and 450mg/kg procyanidin was given to the fifth group by gavage. After 2 hours, the first group was injected subcutaneously with normal saline, the second, third, fourth and the fifth groups were injected subcutaneously with different dose of mercuric chloride (2.2 μmol/kg, 4.4 μmol/kg, 8.8 μmol/kg, 8.8μmol/kg respectively). The treatment were done again on the second day. After 24 hours of the last injection finished, 24-hour urine were collected to determine the content of urinary Hg and the activities of LDH, NAG and ALP. The cortex of kidney was removed to determine the levels of Hg, GSH, MDA, and the activities of SOD and GSH-Px. Blood collected from abdominal aorta was used for determination of BUN in serum. Results In comparison with the control group, the differneces of urinary protein content and Hg, GSH and MDA levels in renal cortex, LDH in urine, and SOD, GSH-px activity in renal cortex in each HgCl2 groups were statistically significant (P<0.05 or P<0.01). Meanwhile, the differences of Hg content and ALP, NAG activities in urine and BUN in serum in 4.4, 8.8 μmol/kg HgCl2 groups were statistically significant (P<0.01). In contrast, compared to 8.8 μmol/kg HgCl2 group, the contents of the urinary protein and BUN in serum decreased, the activities of NAG, LDH and ALP depressed in urine, the levels of GSH and MDA decreased and the activities of SOD and GSH-Px enhanced in kidney in the pretreatment group of procyanidins. The differences between them were statistically significant (P<0.05 or P<0.01). Conclusion Mercuric chloride can accumulate in kidney and cause damage. Procyanidin has a certain protective effect against mercuric chloride-induced renal damage in rat.
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