硫酸铟对小鼠成纤维细胞毒性作用的研究
Effects of Indium Sulfate on Cytotoxicity in L929 Mouse Fibroblasts
-
摘要:
目的 了解硫酸铟对小鼠成纤维细胞(L929)的细胞毒性、DNA损伤及活性氧含量的影响。 方法 以体外培养的L929细胞为研究对象,采用四氮唑盐比色分析法(MTT法)观察不同染毒浓度(1、2、4、8 mmol/L硫酸铟)在不同时间段(2、24、48 h)染毒对L929细胞的毒性作用;采用单细胞凝胶电泳技术(SCGE)检测在2 h作用下,不同浓度(1、2、4 mmol/L硫酸铟)染毒致DNA损伤的情况及活性氧(reactive oxygen species,ROS)含量的变化。 结果 硫酸铟对L929细胞生长具有抑制作用,在各个浓度及时间段内光密度值随染毒浓度增加及染毒时间延长而降低,阴性组与染毒组差异有统计学意义(P<0.05)。单细胞凝胶电泳结果显示,彗星拖尾率、彗尾DNA%、尾长、Olive尾矩随染毒浓度增加而增加,阴性组与染毒组之间的差异有统计学意义(P<0.05),且ROS含量随染毒浓度增加表现递增。 结论 本实验条件下,硫酸铟能够明显抑制L929细胞增殖,存在明显的剂量-效应关系和时间-效应关系,并在短时间内能够引起DNA单链断裂及ROS含量增多。 Abstract:Objective To investigate cytotoxicity, DNA damage and reactive oxygen species (ROS) of fibroblasts of L929 cell line induced by indium sulfate (IS) in vitro. Methods The L929 cells cultured in vitro were exposed to IS at varied dosages (1, 2, 4 and 8 mmol/L) for different exposure periods (2, 24 and 48 h). The cytotoxicity were observed by methyl thiazolyl tetrazolium (MTT) colorimetric assay. Single cell gel electrophoresis (SCGE) was used to detect the DNA damage and ROS concentration of L929 cells after exposed to different concentrations (1, 2 and 4 mmol/L) of IS for 2 h. Results The absorbency of the interferred cells turned lower than that of the control group along with the IS concentration increasing. The difference was significant between the exposed cells and the controls (P<0.05). SCGE results showed that the comet cells ratio, the tail DNA%, the tail length, the Olive tail moment and the concentration of ROS increased with the increasing IS concentrations. The statistical differences among the control group and the various exposed groups were significant (P<0.05). Conclusion Under current experimental conditions, IS presents cytotoxicity on L929 cells in dose-and time-dependent manner, and it can also induce DNA single-strand break (SSB) and increase ROS in a relatively short period of time.
下载:
