一种无磷洗衣粉的消毒效果研究
Evaluation on Disinfection of an Environmental Friendly and Phosphate-free Washing Powder
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摘要:
目的 研究一种新型环保无磷洗衣粉对不同种类细菌的杀灭效果以及对乙型肝炎表面抗原(HBsAg)的破坏作用。 方法 采用载体定量杀灭实验的方法评价浓度为1.0%(w/v)、5.5%(w/v)和10.0%(w/v)的某品牌无磷洗衣粉溶液对大肠埃希氏菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)、枯草杆菌黑色变种(Bacillus subtilis var.neger)芽胞的杀灭效果及其对乙型肝炎表面抗原(HBsAg)的破坏效果。 结果 1.0%的无磷洗衣粉溶液作用10min,对大肠埃希氏菌的杀灭率为(99.90& #177;0.01)%,达到消毒效果要求。10.0%的无磷洗衣粉溶液作用20 min,对金黄色葡萄球菌的杀灭率为(99.92& #177;0.01)%,达到消毒效果;作用40 min,对枯草杆菌黑色变种芽胞的杀灭率仅为(8.06& #177;0.05)%;作用60 min,不能破坏乙型肝炎表面抗原(HBsAg)。 结论 所检测环保无磷洗衣粉的杀菌效果随溶液中洗衣粉浓度的增高以及与微生物作用时间的延长而增强;对大肠杆菌的的杀灭作用明显强于对金黄色葡萄球菌的杀灭作用,而对枯草杆菌黑色变种芽胞的杀灭效果及对乙型肝炎表面抗原(HBsAg)的破坏作用较差。 Abstract:Objective To evaluate the effect of disinfection of a new environmental friendly and phosphate-free washing powder on various microorganisms and surface antigen of hepatitis B virus(HBsAg). Methods Carrier quantitative kill experiment was conducted to determine the effect of disinfection in 1.0%(w/v), 5.5%(w/v), as well as 10.0%(w/v) washing powder solution on Escherichia coli, Staphylococcus aureus, Spores of bacillus subtilis var. neger and HBsAg, respectively. Results The killing rate of 1.0% washing powder solution on E. coli was (99.90& #177;0.01)% during 10 min exposure and the killing rate of 10.0% washing powder solution on S. aureus was (99.90 & #177;0.01)% during 20 min exposure. However, the killing rate of 10.0% washing powder solution on the spores of B. subtilis var. neger was only (8.06 & #177;0.05)% during 40 min exposure, and the HBsAg did not be destroyed during 60 min exposure. Conclusion Generally, the enhanced disinfection effect on microorganisms was obtained with the increasing in the concentration of the tested washing powder solution or the disinfectant contact time. In addition, the tested washing powder solution showed strong bactericidal effects on E. coli than S. aureus, but no significant disinfection effect on the spores of B. subtilis var. neger and HBsAg.
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