袁金华, 黄薇, 查河霞, 宋黎军. 高效液相色谱法同时检测化妆品中7种性激素的条件选择及简化前处理[J]. 环境与职业医学, 2010, 27(8): 509-511,514.
引用本文: 袁金华, 黄薇, 查河霞, 宋黎军. 高效液相色谱法同时检测化妆品中7种性激素的条件选择及简化前处理[J]. 环境与职业医学, 2010, 27(8): 509-511,514.
YUAN Jin-hua , HUANG Wei , ZHA He-xia , SONG Li-jun . Simplified Pretreatment and Simultaneous Determination of Seven Sex Hormones in Cosmetics via High Performance Liquid Chromatography[J]. Journal of Environmental and Occupational Medicine, 2010, 27(8): 509-511,514.
Citation: YUAN Jin-hua , HUANG Wei , ZHA He-xia , SONG Li-jun . Simplified Pretreatment and Simultaneous Determination of Seven Sex Hormones in Cosmetics via High Performance Liquid Chromatography[J]. Journal of Environmental and Occupational Medicine, 2010, 27(8): 509-511,514.

高效液相色谱法同时检测化妆品中7种性激素的条件选择及简化前处理

Simplified Pretreatment and Simultaneous Determination of Seven Sex Hormones in Cosmetics via High Performance Liquid Chromatography

  • 摘要: 目的 简化预处理步骤,提高化妆品中7种性激素检测的准确度、精密度。

    方法 利用乙醇溶解超声分散离心过滤,选择合适的色谱条件,实现7种性激素的完全分离和同时检测。

    结果 实验中的预处理步骤能满足测定需要,确定检测波长为230 nm,选择流动相乙腈:水(40:60,体积比),保留时间<30 min,7种性激素组分完全基线分离,能避开杂质峰的干扰。7种性激素的工作曲线在0.1~50.0 mg/L的范围内呈良好的线性关系,回归方程的相关系数>0.999。回收率为84%~104%,相对标准偏差为1.9%~8.3%,最低检出限0.01~0.06 mg/L。

    结论 按照本实验预处理方法进行测定,可实现7种性激素组分完全基线分离和同时测定。准确度、精密度和检出限均满足实验测定要求。

     

    Abstract: Objective To develop a simplified pretreatment method for simultaneous concentration determination of seven hormones in cosmetics via high performance liquid chromatography (HPLC).

    Methods After a simplified pretreatment of the samples, the proper HPLC conditions were optimized.

    Results This simplified pretreatment was good enough for the measurement. An XTerra TMRP18 column was employed and a mixture of acetonitrile:aqueous (40:60)was used as mobile phase. The measuring wavelength was 230 nm. The proper HLPC conditions were investigated for the determination of seven hormones in the linear range 0.1-50.0 mg/L (correlation coefficients greater than 0.999, and the recoveries for the seven sex hormones ranged from 84%-104% with relative standard deviations of 1.9%-8.3%. The lowest detection limits were 0.01-0.06 mg/L.

    Conclusion The method is suitable for sitmultaneous determination of the seven sex hormones in cosmetics with simplicity and accuracy.

     

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