许洁, 俞捷, 汪洋, 张子萍, 张洋, 吴伟成. 母体暴露壬基酚对仔鼠脾脏组织的氧化损伤[J]. 环境与职业医学, 2010, 27(6): 366-367,370.
引用本文: 许洁, 俞捷, 汪洋, 张子萍, 张洋, 吴伟成. 母体暴露壬基酚对仔鼠脾脏组织的氧化损伤[J]. 环境与职业医学, 2010, 27(6): 366-367,370.
XU Jie , YU Jie , WANG Yang , ZHANG Zi-ping , ZHANG Yang , WU Wei-cheng . Toxicity of Nonylphenol on F1 Rats' Spleen after Gestation Exposure[J]. Journal of Environmental and Occupational Medicine, 2010, 27(6): 366-367,370.
Citation: XU Jie , YU Jie , WANG Yang , ZHANG Zi-ping , ZHANG Yang , WU Wei-cheng . Toxicity of Nonylphenol on F1 Rats' Spleen after Gestation Exposure[J]. Journal of Environmental and Occupational Medicine, 2010, 27(6): 366-367,370.

母体暴露壬基酚对仔鼠脾脏组织的氧化损伤

Toxicity of Nonylphenol on F1 Rats' Spleen after Gestation Exposure

  • 摘要: 目的 研究母体暴露壬基酚(NP)对仔鼠脾脏的氧化损伤。

    方法 将SD母鼠随机分为50、100、200 mg/kgNP暴露组及对照组,每组6~7只动物。在母鼠妊娠第9~15天灌胃NP,仔鼠(F1)于出生60天龄时处死并取脾脏称重。比色法测定脾脏组织中超氧化物歧化酶(SOD)活力及脂质过氧化产物丙二醛(MDA)含量;透射电镜观察其超微结构改变。

    结果 与对照组比较, 200 mg/kg NP组仔鼠的脾脏重量及脏器系数均明显降低(P<0.05),且与染毒剂量呈负相关(r=-0.618, r=-0.530, P<0.05); 100、200mg/kg NP组仔鼠脾脏组织的SOD活力降低, 200mg/kg NP组MDA含量升高(P<0.05),均存在剂量效应关系(rSOD=-0.460, rMDA=0.747, P<0.05)。高暴露组仔鼠脾脏电镜下可见脾细胞线粒体肿胀呈空泡样变,染色质浓缩成块状聚集在核周围。

    结论 NP可透过胎盘屏障致仔鼠脾脏脂质过氧化损伤。

     

    Abstract: Objective To investigate the adverse effects on F1 rats' spleen while the pregnant mother rats were exposed to nonylphenol(NP).

    Methods Pregnant rats were exposed to NP via gavage at doses of 0, 50, 100 and 200 mg/kg by body weights respectively per day, from pregnant days 9 to 15. The level of malondialdehyde (MDA)and activity of super oxide dimutese(SOD) in F1 rats' spleen homogenate were detected respectively at the age of 60th day. The ultrastructure of spleen tissues were observed under electronic microscope.

    Results Compared with the control group, the absolute and relative weight of spleen were decreased dramatically in the 200 mg/kg NP treated group and negative relation between the weight and dose was found (rspleen=-0.618, rcoefficient=-0.530, P<0.05). The SOD activity of spleen tissues decreased in NP 100 and 200 mg/kg exposed fetal rats, while the levels of MDA increased in NP 200 mg/kg exposed fetal rats. Both of them were in a dose-dependent manner(rSOD=-0.460, rMDA=0.747, P<0.05). The differences mentioned above were statistical significant compared to control group (P<0.05). The changes of the ultrastructrue were found among the spleen of NP 200 mg/kg group that characterized with chromatin condesed, clumped in circa-nuclei and mitochondrial tumefaction and vacuolization.

    Conclusion NP can get across the placenta barrier to make the F1 offspring rats' spleen damage of lipid peroxidation.

     

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