细胞间黏附分子-1基因多态性与儿童哮喘患病易感性的关系
Association between Intercellular Adhesion Molecule 1(ICAM-1) Gene Polymorphisms and Genetic Susceptibility on Childhood Asthma
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摘要:
目的 研究细胞间黏附分子-1(ICAM-1)基因Lys469Glu和Arg241Gly位点多态性与儿童哮喘易感性的关系。 方法 对北京某郊区40所小学中的25所进行整群抽样,以1~5年级小学生为研究对象,由家长填写调查问卷。从这些学生中抽取293例病例和668例对照为研究样本,再以问卷调查环境影响因素。采集口腔黏膜细胞提取DNA,采用聚合酶链反应-限制性片段长度多态性方法分析ICAM-1基因多态性,用Logistic回归分析不同基因型与儿童哮喘患病易感性的关系。 结果 ICAM-1 Lys469Glu位点变异基因型(AG或GG)和杂合基因型AG均明显降低患哮喘的危险性(P < 0.05),OR值均为0.73。病例组变异的G等位基因频率为28.5%,对照组为33.0%,两者比较, P=0.058。在有被动吸烟的样本人群中,携带AG或GG基因型的个体患哮喘的危险性是携带AA基因型的个体的0.73倍(95%CI为0.52~1.02, P=0.066)。在烧煤做饭和家中有较多老鼠时,携带AG或GG基因型的人发生哮喘的危险性也明显降低(P < 0.05), OR值分别为0.57(95%CI:0.33~0.97)和0.65(95%CI:0.46~0.93)。未发现ICAM-1基因Arg241Gly多态性与儿童哮喘患病的关联。 结论 ICAM-1 Lys469Glu的变异可能与儿童哮喘易感性有关联,并且降低患哮喘的危险性。 Abstract:Objective To investigate the association between Lys469Glu and Arg241Gly of intercellular adhesion molecule 1(ICAM-1) gene polymorphisms and genetic susceptibility on childhood asthma. Methods Twenty-five primary schools were selected from 40 schools located in a suburban district of Beijing. The parents of the students of 1st~5th grades were asked to complete a questionnaire, then 293 cases and 668 controls were obtained from this study population. The influence factors on asthma were acquired by another questionnaire. DNA samples were extracted from buccal cells. Two single nucleotide polymorphisms(SNPs) in ICAM-1 gene, including Lys469Glu and Arg241Gly were detected by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). The association between genotypes and childhood asthma were analyzed by Logistic regression. Results The genotypes AG or GG and AG of ICAM-1 Lys469Glu decreased the risk of asthma(P < 0.05), the odds ratios (OR) were both 0.73. The frequency of G allele in cases was 28.5%, and that in controls was 33.0%. The former was lower than the latter, which was marginally significant, P=0.058. When there was passive smoking, the children with genotype AG or GG had lower risk of asthma compared with those with genotype AA, OR=0.73 (P=0.066). The decreased risks were also found for those with genotype AG or GG in the subgroup using coal for cooking (OR=0.57, 95%CI 0.33-0.97)and the subgroup having many rats indoors (OR=0.65, 95%CI 0.46-0.93)compared with those with genotype AA. No association was found between Arg241Gly polymorphism and childhood asthma. Conclusion The variation of ICAM-1 Lys469Glu is significantly related to genetic susceptibility on childhood asthma, possibly decreasing the risk of asthma.
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