水母雪莲对运动性疲劳小鼠免疫功能的影响
Experimental Study on Effect of Saussurea Medusa Maxim on Immune Function of Mice with Exercise induced Fatigue
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摘要:
目的 观察水母雪莲对运动性疲劳小鼠免疫功能的影响,探讨其抗运动性疲劳的作用。 方法 选用雄性BALB/C小鼠,随机分成对照组、运动组和运动+雪莲组。运动组和运动+雪莲组采用游泳法致动物慢性力竭性运动性疲劳,然后测定各组免疫功能的变化。 结果 对照组、运动组和运动+雪莲组脾抗体形成细胞(PFC)数分别为75329& #177;378、37569& #177;306和63291& #177;402;脾细胞对刀豆蛋白A (ConA)刺激增殖反应分别为0.975& #177;0.027,0.404& #177;0.053和0.652& #177;0.042;脾脏指数分别为113& #177;12、86& #177;15和102& #177;19;胸腺指数分别为19& #177;7、19& #177;10和17& #177;8。运动组与对照组比较,前3项指标都有明显下降(P<0.01);运动+雪莲组与对照组比较也有下降(P<0.01或P<0.05),但与运动组比较,升高明显(P<0.01或P<0.05)。 结论 水母雪莲能改善实验小鼠免疫功能,具有较好的抗运动性疲劳作用。 Abstract:Objective To observe the effect of Saussurea Medusa Maxim (SMM) on immune function of mice with exercise-induced fatigue and discuss the mechanism of its anti-fatigue. Methods Male BALB/c mice were randomly divided into a control group and 2 experiment groups, i.e. the exercise-induced fatigue group and the exercise-induced fatigue+SMM group. The chronic fatigue model was established by exhaustive swimming. Mice in the control group and in the exercise-induced fatigue group were given through gavage with 0.2 mL water while mice in the exercise-induced fatigue+SMM group with 0.1 g Saussurea Medusa Maxim every day for 14 days. The mice in experiment groups received 7 times exhaustive swimming to induce fatigue during the 15 days. On the 15th day, lymphocyte proliferation induced by ConA, plaque forming cell(PFC)and immune organ index were observed. Results After exhaustive swimming, the lymphocyte proliferation induced by ConA, the plaque forming cells and immune organs index in both experiment groups were lower than those in the control group(P<0.01), while those indices in the exercise-induced fatigue+SMM group were higher than those in the exercise-induced fatigue group(P<0.01). Conclusion Saussurea medusa maxim can obviously improve the immune function of mice with exercise-induced fatigue. It has an effect of resisting exercise fatigue.
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