李金玉, 李艳宁, 邓云珺, 聂继盛. 孕妇产前多环芳烃水平与新生儿脐血淋巴细胞DNA端粒长度的相关性[J]. 环境与职业医学, 2018, 35(9): 809-815. DOI: 10.13213/j.cnki.jeom.2018.18276
引用本文: 李金玉, 李艳宁, 邓云珺, 聂继盛. 孕妇产前多环芳烃水平与新生儿脐血淋巴细胞DNA端粒长度的相关性[J]. 环境与职业医学, 2018, 35(9): 809-815. DOI: 10.13213/j.cnki.jeom.2018.18276
LI Jin-yu, LI Yan-ning, DENG Yun-jun, NIE Ji-sheng. Association between maternal prenatal polycyclic aromatic hydrocarbons level and newborns' DNA telomere length in cord blood lymphocytes[J]. Journal of Environmental and Occupational Medicine, 2018, 35(9): 809-815. DOI: 10.13213/j.cnki.jeom.2018.18276
Citation: LI Jin-yu, LI Yan-ning, DENG Yun-jun, NIE Ji-sheng. Association between maternal prenatal polycyclic aromatic hydrocarbons level and newborns' DNA telomere length in cord blood lymphocytes[J]. Journal of Environmental and Occupational Medicine, 2018, 35(9): 809-815. DOI: 10.13213/j.cnki.jeom.2018.18276

孕妇产前多环芳烃水平与新生儿脐血淋巴细胞DNA端粒长度的相关性

Association between maternal prenatal polycyclic aromatic hydrocarbons level and newborns' DNA telomere length in cord blood lymphocytes

  • 摘要: 目的 研究孕妇产前多环芳烃暴露与新生儿脐血淋巴细胞DNA端粒长度的相关性。

    方法 在太原市2家医院共招募347名孕妇,签署知情同意书。在产前1周内收集孕妇尿液于-80℃保存待检。在分娩时收集新生儿脐带血并提取淋巴细胞DNA。应用高效液相色谱法测定孕妇尿中4种多环芳烃代谢物(2-羟基萘、2-羟基芴、9-羟基菲和1-羟基芘)。应用实时荧光定量PCR测定新生儿脐血淋巴细胞DNA端粒长度。采用方差分析、t'检验及Spearman相关进行相对端粒长度的单因素分析;采用协方差分析探究PAHs代谢物与端粒长度的剂量反应关系;采用多元线性回归分析4种多环芳烃代谢物与新生儿脐血淋巴细胞DNA端粒长度的关系。

    结果 孕妇尿中2-羟基萘、2-羟基芴、9-羟基菲和1-羟基芘水平的中位数分别为7.56、3.89、3.42、1.55 μg/g(以肌酐校正)。新生儿脐血淋巴细胞DNA相对端粒长度的中位数(P25~P75)为0.85(0.61~1.31)。随着孕妇尿液中2-羟基芴(P=0.022)和1-羟基芘(P=0.033)水平的升高,脐血淋巴细胞DNA端粒长度有下降趋势。控制相关混杂因素后,孕妇尿中2-羟基芴和1-羟基芘水平每增加一个自然倍数,新生儿脐血淋巴细胞DNA端粒长度平均缩短12.19%、8.61%。

    结论 产前多环芳烃暴露与新生儿脐血淋巴细胞DNA端粒长度的缩短有关。

     

    Abstract: Objective To evaluate the association between maternal prenatal exposure to polycyclic aromatic hydrocarbons and newborns' DNA telomere length in cord blood lymphocytes.

    Methods A total of 347 pregnant women were recruited from two hospitals in Taiyuan and provided written informed content. Their urinary samples were collected a week before delivery and were stored at -80℃. Newborns' cord blood samples were collected at delivery to extract DNA from lymphocytes. Mothers' urinary concentrations of four PAH metabolites, including 2-hydroxynaphthalene (2-OH NAP), 2-hydroxyfluorene (2-OH FLU), 9-hydroxyphenanthrene (9-OH PHE), and 1-hydroxypyrene (1-OH PYR), were detected by high performance liquid chromatography. DNA telomere lengths in cord blood lymphocytes of newborns were measured by real-time fluorescence quantitative PCR. Univariate analysis on telomere length included one-way ANOVA, t' test, and Spearman correlation analysis; analysis of covariance was used to assess the dose-response association between PAH metabolites and telomere length; multiple linear regression analysis was used to evaluate the association between four maternal urinary PAH metabolites and newborns' DNA telomere length in cord blood lymphocytes.

    Results The median concentrations of 2-OH NAP, 2-OH FLU, 9-OH PHE, and 1-OH PYR in the mothers' urine samples were 7.56, 3.89, 3.42, and 1.55 μg/g (adjusted for creatinine), respectively. The median (P25-P75) of relative DNA telomere length in the newborns' cord blood lymphocytes was 0.85 (0.61-1.31). With 2-OH FLU and 1-OH PYR concentrations increasing in mothers' urine samples, newborns' DNA telomere length in cord blood lymphocytes showed decreasing trends (P=0.022, P=0.033). Each lnunit increase in urinary 2-OH FLU and 1-OH PYR was associated with 12.19% and 8.16% decrease in DNA telomere length in cord blood lymphocytes after adjusting for potential confounders.

    Conclusion Maternal PAHs exposures before delivery are associated with decreased newborns' DNA telomere length in cord blood lymphocytes.

     

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