介入放射诊疗工作人员机体DNA甲基化状态与职业暴露的关系
Relationship between DNA methylation and occupational exposure among radiologists engaged in interventional treatment
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摘要:
目的 探讨介入放射诊疗工作人员职业暴露对机体DNA甲基化状态、甲基化相关酶类及甲基化次生产物的影响。
方法 暴露组为120名放射工龄3年以上的介入放射诊疗工作人员，对照组为性别相同、年龄±3岁的120名非放射诊疗医技人员。采集空腹静脉血，前处理后通过高效液相色谱法和酶标法依次检测DNA总甲基化率、DNA甲基化转移酶（DNA methyltransferase，Dnmt）和同型半胱氨酸（homocysteine，Hcy）的表达水平并比较两组差异。采用偏相关和协方差分析暴露组人群DNA甲基化指标与年有效剂量监测值和介入工龄的关系。采用多元线性回归分析个人剂量监测值、介入工龄、年龄等因素对机体甲基化状态的影响。
结果 暴露组Dnmt为（0.064±0.043）×103 U/L，Hcy为（10.37±3.92）μmol/L，均高于对照组（0.047±0.035）×103 U/L和（9.32±3.90）μmol/L（P < 0.05），但DNA总甲基化率暴露组（8.10±1.53）%，对照组（8.47±1.41）%组间差异无统计学意义（P > 0.05）。暴露组年有效剂量值与Dnmt呈正相关（r=0.671，P=0.00），未发现其他指标与年累积个人剂量值存在相关性。多因素分析显示，年龄和介入工龄（标准化回归系数分别为-0.182和-0.298，均P < 0.01）是影响介入工作者机体甲基化状态的重要因素。
结论 介入放射诊疗工作者职业暴露对机体DNA甲基化过程中的相关酶类及代谢产物的表达存在一定影响，但未见对DNA甲基化状态的影响。
Abstract:Objective To evaluate the effects of occupational exposure to radiation during interventional procedures on DNA methylation, DNA methylation related enzymes, and methylation metabolites.
Methods The exposure group was composed of 120 radiologists who had been engaged in interventional treatment for more than three years, and the control group included 120 non-interventional radiologists matched for sex and age (±threes years) with the exposure group. Total methylation rate of DNA and expression levels of DNA methyltransferase (Dnmt) and homocysteine (Hcy) in fasting venous blood samples were detected and compared by high performance liquid chromatography and enzyme-linked assay, respectively. The relationships of DNA methylation related indicators with annual effective individual dose and working age for interventional radiology were analyzed by partial correlation analysis and analysis of covariance. The effects of individual dose, working age for interventional radiology, age, and other factors on the methylation levels of the subjects were analyzed by multi-factor linear regression.
Results The expression levels of Dnmt(0.064±0.043) U/mL and Hcy(10.37±3.92) μmol/L in the exposure group were higher than those in the control group(0.047±0.035)×103 U/L and (9.32±3.90) μmol/L (P < 0.05); however, the total DNA methylation rate was not statistically different between the two groupsexposure group:(8.10±1.53)% vs control group:(8.47±1.41)% (P > 0.05). Annual effective individual dose in the exposure group was positively correlated with Dnmt (r=0.671, P=0.00), but not correlated with other indicators. The results of multi-factor analysis showed that age and working age for interventional radiology were important factors affecting DNA methylation levels of interventional radiologists (standardized partial regression coefficients were -0.182 and -0.198, respectively, Ps < 0.01).
Conclusion Occupational exposure to interventional treatment relevant radiation may affect the expression of related enzymes and metabolites in the process of DNA methylation, but has little effect on the DNA methylation of interventional radiologists.
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