任冬燕, 徐梦伟, 孙高峰, 谢惠芳. 大鼠砷染毒与糖尿病的相关性[J]. 环境与职业医学, 2018, 35(2): 102-107. DOI: 10.13213/j.cnki.jeom.2018.17602
引用本文: 任冬燕, 徐梦伟, 孙高峰, 谢惠芳. 大鼠砷染毒与糖尿病的相关性[J]. 环境与职业医学, 2018, 35(2): 102-107. DOI: 10.13213/j.cnki.jeom.2018.17602
REN Dong-yan, XU Meng-wei, SUN Gao-feng, XIE Hui-fang. Association between arsenic exposure and diabetes mellitus in rats[J]. Journal of Environmental and Occupational Medicine, 2018, 35(2): 102-107. DOI: 10.13213/j.cnki.jeom.2018.17602
Citation: REN Dong-yan, XU Meng-wei, SUN Gao-feng, XIE Hui-fang. Association between arsenic exposure and diabetes mellitus in rats[J]. Journal of Environmental and Occupational Medicine, 2018, 35(2): 102-107. DOI: 10.13213/j.cnki.jeom.2018.17602

大鼠砷染毒与糖尿病的相关性

Association between arsenic exposure and diabetes mellitus in rats

  • 摘要: 目的 利用SD大鼠探讨砷染毒与糖尿病的关联性。

    方法 将40只SPF级SD大鼠,随机分为对照组,糖尿病模型组(糖尿病组),亚砷酸钠低、中、高剂量染毒组(低砷组、中砷组、高砷组),每组8只,雌雄各半。对染毒组大鼠连续经口染毒15周,剂量从低到高依次为0.45、2.25、11.25 mg/kg。实验期间每周测定各组大鼠体重;第5周后给糖尿病组大鼠腹腔注射四氧嘧啶建立糖尿病SD大鼠模型。每2周测定1次各组大鼠空腹血糖、尿糖。实验第15周,腹腔麻醉后摘取各组大鼠的心、肝、脾、肺、肾等脏器组织称重;用COD-PAP酶法测定大鼠血样中总胆固醇、三酰甘油,酶标仪直接检测法测定低密度脂蛋白胆固醇、高密度脂蛋白胆固醇,酶比色法测定糖化血红蛋白,ELISA法测定胰岛素、胰岛素样生长因子-1。

    结果 实验第15周时,糖尿病组与高砷组大鼠体重低于对照组(P < 0.05),各染毒组与糖尿病组大鼠的体重差异无统计学意义(P > 0.05)。糖尿病组与高砷组大鼠的肝、肾脏脏器系数均高于对照组(P < 0.05),高砷组与糖尿病组大鼠的肝脏脏器系数差异无统计学意义(P > 0.05)。实验第11、13、15周时高砷组大鼠空腹血糖水平均高于对照组,第15周时高砷组大鼠尿糖检测结果均为阳性(P < 0.05)。与对照组比较,糖尿病组与各染毒组大鼠总胆固醇、三酰甘油、低密度脂蛋白胆固醇和糖化血红蛋白水平明显升高,胰岛素、胰岛素样生长因子-1与高密度脂蛋白胆固醇水平则下降(P < 0.05);高砷组与糖尿病组大鼠的低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、胰岛素、胰岛素样生长因子-1水平差异均无统计学意义(P > 0.05)。

    结论 砷染毒SD大鼠血脂代谢异常,血糖水平升高,高砷组大鼠的糖脂代谢指标变化倾向于糖尿病组,提示砷染毒与糖尿病的发生可能相关。

     

    Abstract: Objective To assess the association between arsenic exposure and diabetes mellitus in SD rats.

    Methods Forty specific-pathogen-free (SPF) SD rats were randomly divided by weight into control group, diabetes mellitus model group (diabetes mellitus group), and low, medium, and high dose arsenic groups, respectively, with eight rats in each group, half male and half female. The arsenic groups were continuously orally treated with sodium arsenite at 0.45, 2.25, and 11.25 mg/kg, respectively, for 15 weeks. During the experiment, the body weight was measured weekly. After 5 weeks of experiment, intraperitoneal injection of alloxan was given to the rats labelled as diabetes mellitus model. Fasting blood glucose and urine glucose were determined once every two weeks. After 15 weeks of experiment, the rats received intraperitoneal anesthesia to collect and weigh heart, liver, spleen, lung, and kidney. Total cholesterol and triglyceride in rat blood samples were determined by COD-PAP method; low-density lipoprotein cholesterol and high-density lipoprotein cholesterol by direct detection using microplate reader; glycosylated hemoglobin by enzyme colorimetric assay; insulin and insulin-like growth factor-1 by ELISA.

    Results In week 15 of the experiment, the weights of the diabetes mellitus group and the high arsenic group were lower than that of the control group (P < 0.05), and there was no significant difference in weight between the arsenic groups and the diabetes mellitus group (P > 0.05). The organ coefficients of liver and kidney of the diabetes mellitus group and the high arsenic group were higher than those of the control group (P < 0.05), but no difference in liver's organ coefficient was observed between the high arsenic group and the diabetes mellitus group (P > 0.05). The average levels of fasting blood glucose of the high arsenic group in week 11, 13, and 15 were higher than those of the control group, and in week 15 positive results for urine glucose were detected in the high arsenic group (P < 0.05). Compared with the control group, the levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol, and glycosylated hemoglobin were significantly increased, whereas insulin, insulin-like growth factor-1, and high-density lipoprotein cholesterol decreased in the diabetes mellitus group and the arsenic groups (P < 0.05). There were no differences in the le vels of low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, insulin, and insulin-like growth factor-1 between the high arsenic group and the diabetes mellitus group (P > 0.05).

    Conclusion Abnormal lipid metabolism and elevated blood glucose are observed in the SD rats exposed to arsenic. The change of glycolipid metabolism index of the high arsenic group is similar to that of the diabetes mellitus group, suggesting that arsenic exposure may be closely related to the occurrence of diabetes.

     

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