李玉洁, 贾晓民, 赵杰. 褪黑素对矽肺大鼠肺纤维化的影响[J]. 环境与职业医学, 2017, 34(7): 642-647. DOI: 10.13213/j.cnki.jeom.2017.17101
引用本文: 李玉洁, 贾晓民, 赵杰. 褪黑素对矽肺大鼠肺纤维化的影响[J]. 环境与职业医学, 2017, 34(7): 642-647. DOI: 10.13213/j.cnki.jeom.2017.17101
LI Yu-jie, JIA Xiao-min, ZHAO Jie. Effect of melatonin on silica induced lung fibrosis in rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(7): 642-647. DOI: 10.13213/j.cnki.jeom.2017.17101
Citation: LI Yu-jie, JIA Xiao-min, ZHAO Jie. Effect of melatonin on silica induced lung fibrosis in rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(7): 642-647. DOI: 10.13213/j.cnki.jeom.2017.17101

褪黑素对矽肺大鼠肺纤维化的影响

Effect of melatonin on silica induced lung fibrosis in rats

  • 摘要: 目的 研究褪黑素(melatonin,MT)对矽肺大鼠肺纤维化的影响及其可能机制。

    方法 将72只SPF级SD雄性大鼠,随机分为4组(对照组、MT组、染尘组及MT+染尘组),每组18只。对照组及MT组采用气管插管灌注法,一次性气管内注入1 mL生理盐水,其他两组采用同样方法注入50 mg/mL的二氧化硅悬液1 mL。每天给予MT组、MT+染尘组腹腔注射MT(10 mg/kg),而染尘组及对照组给予腹腔注射同体积生理盐水。每组分别于染毒后第7、14、28天随机处死6只大鼠。观察大鼠的肺脏器系数、肺组织形态,测定肺组织羟脯氨酸(Hyp)含量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、肺组织核转录因子-κB(NF-κB)的表达。

    结果 MT+染尘组大鼠肺泡炎症细胞浸润、充血水肿、肺间质纤维化的程度较染尘组相比有明显改善;与同期染尘组大鼠相比,MT+染尘组大鼠肺脏器系数、Hyp含量、MDA含量,SOD活性增加,差异均有统计学意义(均P < 0.05)。Western blot检测结果显示,MT+染尘组大鼠肺组织NF-κB蛋白表达量(0.36±0.02)低于染尘组(0.48±0.01),差异有统计学意义(P < 0.05)。

    结论 褪黑素对矽肺大鼠肺纤维化有保护作用,其作用机制可能是通过改善机体氧化/抗氧化系统的失衡、抗炎,从而减少纤维化的形成。

     

    Abstract: Objective To test the potential effect and mechanism of melatonin (MT) on silica induced pulmonary fibrosis in rats.

    Methods Totally 72 specific-pathogen-free SD male rats were randomly divided into four groups (n=18 for each group):control, MT, dust exposure, and MT+dust exposure groups. The rats in the control group and the MT group were injected with 1 mL saline by intratracheal instillation, and those in the other two groups were injected with 1 mL silica suspension (50 mg/mL) by in tratracheal instillation. After administration of silica or saline, the MT group and the MT+dust exposure group were additionally in traperitoneally injected with MT (10 mg/kg), while the control group and the dust exposure group were intraperitoneally injected with saline (10 mL/kg) every day. Six rats of each group were sacrificed 7, 14, and 28 days after exposure. Pulmonary organ coefficient was calculated and lung histomorphology was observed for each group. Hydroxyproline (Hyp) content, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and the expression of nuclear factor kappa B (NF-κB) protein in lung tissues were evaluated.

    Results The MT+ dust exposure group showed reduced lung inflammation, congestion, edema, and interstitial fibrosis compared with the dust exposure group. Compared with the dust exposure group at the same stage, the pulmonary organ coefficients, Hyp content, and MDA content were decreased, while the SOD activity was increased in the MT+dust exposure group (Ps < 0.05). Western blot analysis results showed that the expression of NF-κB protein (0.36±0.02) in lung tissue of the MT+dust exposure group was lower than that of the dust exposure group (0.48±0.01) (P < 0.05).

    Conclusion MT has a protective effect on silica induced lung fibrosis in rats. Its mechanism may be related to the improvement of oxidant/antioxidant system imbalance and anti-inflammatory, thus reducing fibrosis.

     

/

返回文章
返回