陈庆, 赵宗霞, 赵金燕, 李向红, 卢晓宁, 薛翔. 百草枯对雄性大鼠精液质量及睾丸组织脂质过氧化的影响[J]. 环境与职业医学, 2017, 34(4): 337-340. DOI: 10.13213/j.cnki.jeom.2017.16656
引用本文: 陈庆, 赵宗霞, 赵金燕, 李向红, 卢晓宁, 薛翔. 百草枯对雄性大鼠精液质量及睾丸组织脂质过氧化的影响[J]. 环境与职业医学, 2017, 34(4): 337-340. DOI: 10.13213/j.cnki.jeom.2017.16656
CHEN Qing, ZHAO Zong-xia, ZHAO Jin-yan, LI Xiang-hong, LU Xiao-ning, XUE Xiang. Effects of paraquat on semen quality and lipid peroxidation in testis of male rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(4): 337-340. DOI: 10.13213/j.cnki.jeom.2017.16656
Citation: CHEN Qing, ZHAO Zong-xia, ZHAO Jin-yan, LI Xiang-hong, LU Xiao-ning, XUE Xiang. Effects of paraquat on semen quality and lipid peroxidation in testis of male rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(4): 337-340. DOI: 10.13213/j.cnki.jeom.2017.16656

百草枯对雄性大鼠精液质量及睾丸组织脂质过氧化的影响

Effects of paraquat on semen quality and lipid peroxidation in testis of male rats

  • 摘要: 目的 研究百草枯(paraquat,PQ)对雄性大鼠精液质量和睾丸组织脂质过氧化的影响。

    方法 将32只健康成年雄性Wistar大鼠随机分为4组,即对照组和低、中、高三个PQ染毒组(0.5、2.0、8.0mg/kg),每组8只。采用经口灌胃,连续染毒8周。染毒结束后,处死大鼠,分离睾丸及附睾组织,称重并计算脏器系数,分析精子数量及活力,采用H-E染色观察睾丸组织病理学变化,并检测睾丸组织中丙二醛(MDA)含量、超氧化物岐化酶(SOD)以及谷胱甘肽过氧化物酶(GSH-Px)活力。

    结果 低、中、高染毒组大鼠精子数量分别为(48.37±3.76)×106、(41.56±3.13)×106、(32.48±4.09)×106,活力分别为(68.85±5.27)%、(66.65±2.83)%、(52.30±1.99)%,均低于对照组(53.19±4.15)×106,(70.65±6.07)%,差异有统计学意义(P < 0.05)。各染毒组大鼠睾丸脏器系数(0.87±0.08、0.85±0.07、0.78±0.07)及附睾脏器系数(0.27±0.02、0.29±0.03、0.31±0.03)与对照组(0.94±0.06,0.24±0.03)相比较,差异亦具有统计学意义(P < 0.05)。与对照组相比,染毒组睾丸组织出现明显的退行性改变;且高PQ染毒组睾丸组织中MDA含量升高(1.45±0.18)nmol/mg vs.(0.79±0.11)nmol/mg,SOD(76.19±6.71)μ/mg vs.(108.50±11.24)μ/mg及GSH-Px(15.07±2.71)μ/mg vs.(30.61±3.23)μ/mg活力下降。

    结论 百草枯导致大鼠精液质量下降,睾丸组织脂质过氧化增强。

     

    Abstract: Objective To study the effects of paraquat (PQ) on semen quality and lipid peroxidation in testis of male rats.

    Methods Healthy adult male Wistar rats (n=32) were randomly divided into four groups, including control group and paraquat (0.5, 2.0, and 8.0 mg/kg) groups, with eight rats in each group. Animals were exposed to designed treatments by oral gavage for eight weeks and then sacrificed. Testis and epididymis were isolated and weighed, and organ coefficient was calculated. Sperm count and motility plus testes histopathological changes using HE staining were determined. Additionally, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-Px) activity of testis tissues were detected.

    Results The sperm counts (48.37±3.76)×106, (41.56±3.13)×106, and (32.48±4.09)×106 and mobility (68.85±5.27)%, (66.65±2.83), and (52.30±1.99)% of the 0.5, 2.0, and 8.0 mg/kg paraquat groups were less than those of the control group (53.19± 4.15)×106 and (70.65±6.07)% (P < 0.05) respectively. The organ coefficients of testis (0.87±0.08, 0.85±0.07, and 0.78±0.07) and epididymis (0.27±0.02, 0.29±0.03, and 0.31±0.03) of the paraquat groups were different from those of the control group (0.94±0.06 and 0.24±0.03) (P < 0.05) respectively. Obvious degenerative changes were observed in the paraquat treated rats. The 8.0 mg/kg paraquat exposure led to a significant increase in the MDA content (1.45±0.18) nmol/mg vs.(0.79±0.11) nmol/mg, and decrease in the SOD (76.19±6.71) μ/mg vs.(108.50±11.24) μ/mg and GSH-Px (15.07±2.71) μ/mg vs.(30.61±3.23) μ/mg activities of testes tissues.

    Conclusion Paraquat can result in decreased semen quality of rats and elevated lipid peroxidation in testis tissues.

     

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