常志敏, 刘园, 张晓星, 蔚岩, 曹福源, 李清钊, 刘楠, 关维俊. 铟锡氧化物对大鼠体内铁、锌、铜元素含量的影响[J]. 环境与职业医学, 2017, 34(2): 160-164. DOI: 10.13213/j.cnki.jeom.2017.16437
引用本文: 常志敏, 刘园, 张晓星, 蔚岩, 曹福源, 李清钊, 刘楠, 关维俊. 铟锡氧化物对大鼠体内铁、锌、铜元素含量的影响[J]. 环境与职业医学, 2017, 34(2): 160-164. DOI: 10.13213/j.cnki.jeom.2017.16437
CHANG Zhi-min, LIU Yuan, ZHANG Xiao-xing, YU Yan, CAO Fu-yuanb, LI Qing-zhao, LIU Nan, GUAN Wei-jun. Effects of indium-tin oxide on iron, zinc, and copper levels in rats in vivo[J]. Journal of Environmental and Occupational Medicine, 2017, 34(2): 160-164. DOI: 10.13213/j.cnki.jeom.2017.16437
Citation: CHANG Zhi-min, LIU Yuan, ZHANG Xiao-xing, YU Yan, CAO Fu-yuanb, LI Qing-zhao, LIU Nan, GUAN Wei-jun. Effects of indium-tin oxide on iron, zinc, and copper levels in rats in vivo[J]. Journal of Environmental and Occupational Medicine, 2017, 34(2): 160-164. DOI: 10.13213/j.cnki.jeom.2017.16437

铟锡氧化物对大鼠体内铁、锌、铜元素含量的影响

Effects of indium-tin oxide on iron, zinc, and copper levels in rats in vivo

  • 摘要: 目的 探讨铟锡氧化物(ITO)对大鼠体内铁、锌、铜元素含量的影响。

    方法 选取SPF级Wistar雄性大鼠24只,随机分为对照组、ITO 3 mg/kg组和ITO 6 mg/kg组。对染毒组大鼠进行肺灌注染毒,每周2次,连续染毒8周。染毒结束后,在屏障环境下继续喂养8周。造模结束后立即处死大鼠,摘取其肺脏、肝脏、肾脏、心脏、脾脏和股骨,经微波消解处理后,采用电感耦合等离子体质谱法测定铟、铁、锌、铜元素含量。

    结果 与对照组相比,ITO 3 mg组和6 mg组大鼠肺脏、肝脏、心脏、脾脏、股骨铟含量均更高(均P<0.05),肺脏、肾脏中锌含量更低(均P<0.05),肺脏、肝脏中铁含量更低(均P<0.05);ITO 6 mg组大鼠肺脏、肝脏和肾脏中铜/锌值均高于对照组(P<0.05)。

    结论 ITO染毒可降低大鼠肺脏组织中锌、铁含量,使铜/锌值升高,进而干扰大鼠体内微量元素的平衡。

     

    Abstract: Objective To test the changes of iron, zinc, and copper levels in rats exposed to indium-tin oxide (ITO).

    Methods SPF Wistar male rats (n=24) were randomly assigned into three groups and intratracheally administered with 0, 3, or 6 mg/kg of ITO solution, twice a week, for eight weeks. The rats were euthanized serially up to eight weeks after the final instillation and followed by collecting samples of lung, liver, kidney, heart, spleen, and thighbone. After microwave digestion, inductively coupled plasma mass spectrometry were used to determine the levels of indium, iron, zinc, and copper.

    Results Compared with the control group, the indium levels in lung, liver, heart, spleen, and thighbone in the ITO 3 mg/kg group and 6 mg/kg group were higher (Ps<0.05); the zinc levels in lung and kidney of the two exposed groups were lower (Ps<0.05); the iron levels in lung and liver of the two exposed groups were also lower (Ps<0.05). The copper/zinc ratios in lung, liver, and kidney of the ITO 6 mg/kg group were higher than that of the control group (P<0.05).

    Conclusion After ITO exposure, the levels of zinc and iron in lung are reduced, and the copper/zinc ratio is increased, indicating imbalanced trace elements.

     

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