后肢接振对家兔神经生长因子及其受体的影响
Effects on nerve growth factor and nerve growth factor receptor in rabbits exposed to vibration by hind legs
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摘要:
目的 研究后肢接振对家兔血清及组织中神经生长因子与受体的影响。
方法 将32只新西兰兔随机分为低剂量组、中剂量组、高剂量组3个实验组和1个对照组。3个实验组的接振剂量（4 h等能量频率计权加速度）分别为4.33、8.67、17.34 m/s2进行接振试验，对照组只接触与中剂量组相同的噪声声级。接振试验结束后，用酶联免疫法测定家兔血清及组织中神经生长因子及受体的浓度。
结果 与对照组比较，中剂量组、高剂量组血清及组织中神经生长因子的含量均升高（P < 0.05，P < 0.01），中剂量组、高剂量组组织中神经生长因子受体含量均升高（P < 0.01）。
结论 后肢接振所致家兔血清及组织中神经生长因子与受体浓度的升高，可能与振动性神经损伤的发生有一定联系。
Abstract:Objective To study the effects on nerve growth factor and nerve growth factor receptor in serum and tissue of rabbits exposed to vibration by hind legs.
Methods Thirty-two New Zealand rabbits were randomly divided into three experimental groups (low-dose, middle-dose, and high-dose) and one control group. The experimental groups were tested with vibration intensities of (4-hour energy equivalent frequency-weighted acceleration) 4.33 m/s2 (low-dose group), 8.67 m/s2 (middle-dose group), and 17.34 m/s2 (high-dose group), respectively. The control group was only exposed to noise at the same sound level as the middle-dose group. The concentrations of nerve growth factor and its receptor in serum and tissue were measured by enzyme linked immunosorbent assay after vibration test.
Results Compared with the control group, the concentrations of nerve growth factor in serum and tissue increased (P < 0.05, P < 0.01), and the concentrations of nerve growth factor receptor in tissue increased significantly in the middle-dose group and the highdose group (P < 0.01).
Conclusion Vibration by hind legs could result in concentrations increment of nerve growth factor and nerve growth factor receptor in serum and tissue, which may be related to nervous impairment induced by vibration.
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