张秀峰, 朱丽, 刘舒鹏, 候晓敏, 陈紫莺, 刘微, 郝小惠, 王宏丽, 刘和亮. 矽肺患者肺泡巨噬细胞中胆固醇及ox-LDL水平分析[J]. 环境与职业医学, 2016, 33(12): 1157-1161. DOI: 10.13213/j.cnki.jeom.2016.16523
引用本文: 张秀峰, 朱丽, 刘舒鹏, 候晓敏, 陈紫莺, 刘微, 郝小惠, 王宏丽, 刘和亮. 矽肺患者肺泡巨噬细胞中胆固醇及ox-LDL水平分析[J]. 环境与职业医学, 2016, 33(12): 1157-1161. DOI: 10.13213/j.cnki.jeom.2016.16523
ZHANG Xiu-feng, ZHU Li, LIU Shu-peng, HOU Xiao-min, CHEN Zi-ying, LIU Wei, HAO Xiaohui, WANG Hong-li, LIU He-liang. Analysis of Cholesterol and Ox-LDL Levels in Alveolar Macrophages from Patients with Silicosis[J]. Journal of Environmental and Occupational Medicine, 2016, 33(12): 1157-1161. DOI: 10.13213/j.cnki.jeom.2016.16523
Citation: ZHANG Xiu-feng, ZHU Li, LIU Shu-peng, HOU Xiao-min, CHEN Zi-ying, LIU Wei, HAO Xiaohui, WANG Hong-li, LIU He-liang. Analysis of Cholesterol and Ox-LDL Levels in Alveolar Macrophages from Patients with Silicosis[J]. Journal of Environmental and Occupational Medicine, 2016, 33(12): 1157-1161. DOI: 10.13213/j.cnki.jeom.2016.16523

矽肺患者肺泡巨噬细胞中胆固醇及ox-LDL水平分析

Analysis of Cholesterol and Ox-LDL Levels in Alveolar Macrophages from Patients with Silicosis

  • 摘要: 目的

    分析不同期别矽肺患者肺泡灌洗液(BALF)及肺泡巨噬细胞中胆固醇及氧化低密度脂蛋白(ox-LDL)水平的变化,探讨脂质代谢在矽肺纤维化进展过程中的作用。

    方法

    选取59例男性矽肺患者,其中壹期18例,贰期17例,叁期24例,对照组8例,另选取12名健康成年人作为血清对照组。应用HE染色和油红O染色观察肺泡巨噬细胞形态和脂质蓄积情况;应用ELISA法检测BALF和肺泡巨噬细胞中胆固醇、ox-LDL和血清中人结缔组织生长因子(CTGF)水平。

    结果

    肺泡巨噬细胞中有脂质存在,随着矽肺分期增高,肺泡巨噬细胞和肺泡灌洗液中胆固醇含量均呈上升趋势,矽肺叁期组均明显高于对照组、矽肺壹期组、矽肺贰期组,差异具有统计学意义(P<0.05);肺泡巨噬细胞中ox-LDL水平随矽肺分期呈上升趋势,而BALF中ox-LDL水平呈下降趋势,组间两两比较差异具有统计学意义(P<0.05)。与血清对照组相比,矽肺患者血清中CTGF含量随矽肺进展不断升高(P<0.05);肺泡巨噬细胞中胆固醇、ox-LDL及BALF中胆固醇与CTGF呈正相关(P<0.05),BALF中ox-LDL与CTGF呈负相关(P<0.05)。

    结论

    在矽肺纤维化发展进程中,矽肺患者肺泡巨噬细胞中胆固醇代谢失衡,可能与ox-LDL水平增高导致巨噬细胞泡沫化有关。

     

    Abstract: Objective

    To investigate the role of lipid metabolism in the fibrosis progression of silicosis through detecting cholesterol and oxidized low density lipoprotein (ox-LDL) levels in alveolar macrophages and bronchoalveolar lavage fluid (BALF) from patients in different silicosis stages.

    Methods

    Fifty-nine male patients with silicosis were selected,among whom 18 cases were graded as stage Ⅰ,17 cases as stage Ⅱ,and 24 cases as stage Ⅲ;8 controls were recruited;another 12 healthy adults were selected as serum control group.HE staining and oil red O staining were used to observe macrophage morphology and lipid accumulation,and ELISA was used to detect cholesterol,ox-LDL,and connective tissue growth factor (CTGF) levels in alveolar macrophages and BALF.

    Results

    Lipids existed in alveolar macrophages.Cholesterol levels increased in alveolar macrophages and BALF with higher silicosis stages,and the cholesterol level of stage Ⅲ group was significantly higher than that of the control,stage I,and stage Ⅱ groups (P<0.05).The ox-LDL level in alveolar macrophages increased with higher silicosis stages,whereas in the BALF it showed a declining trend (P<0.05).Compared with the serum control group,the CTGF level in serum was increasing with the advance of silicosis (P<0.05).Cholesterol in alveolar macrophages and BALF and ox-LDL in alveolar macrophages were positively correlated with CTGF (P<0.05),but ox-LDL in BALF was negatively correlated with CTGF (P<0.05).

    Conclusion

    In the advance of silicosis fibrosis,cholesterol metabolism disorder in alveolar macrophages are identified in silicosis patients,which might be related to the macrophage foam cells caused by elevated ox-LDL level.

     

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