微小RNA-30c、196a2基因多态性与宫颈癌关系的病例-对照研究
Case-Control Study on Relationship Between Polymorphisms of MicroRNA-30c and MicroRNA-196a2 and Cervical Cancer
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摘要:
目的 探讨微小RNA（miR）-30c 和miR-196a2 基因多态性与宫颈癌的相关性。
方法 于2012 年9 月-2014 年9 月以病例- 对照研究的方法收集来自盐城市第一人民医院的104 例宫颈癌患者及186 例对照，采用多重聚合酶链反应（PCR）和多重连接酶检测反应（LDR）技术对病例组和对照组的miR-30c rs928508 和miR-196a2 rs11614913 基因多态性进行分析，采用卡方检验分析各基因型与宫颈癌发生之间的相关性。
结果 miR-30c rs928508
GG 、AG 和AA 基因型在病例组和对照组中的分别为16.3%、51.9%、31.8%和29.0%、47.3%、23.7%，差异具有统计学意义（P <0.05）。与GG 基因型比较，携带AG 和AA 基因型的个体患宫颈癌的风险明显升高（P <0.05），其中携带AA 基因型个体危险度增加至2.38（95%CI ：1.17~4.83）。未发现miR-196a2 rs11614913 多态性与宫颈癌发生具有相关性（P >0.05）。结论 miR-30crs928508 基因多态性与宫颈癌发生相关。
Abstract:Objective To assess the relationship between polymorphisms of MicroRNA(miR)-30c and miR-196a2 and cervical cancer.
Method A case-control design was carried out in this study, which included 104 patients with cervical cancer and 186 controls from Yancheng City No.1 People's Hospital from September 2012 to September 2014. The gene polymorphisms of miR-30c rs928508 and miR-196a2 rs11614913 in patients and controls were analyzed by multiplex polymerase chain reaction and multiple ligase detection reaction. The association between identified genotypes and cervical cancer was analyzed by chi-square test.
Result The genotype of miR-30c rs928508 were 16.3% for
GG , 51.9% forAG , and 31.8% forAA in the cases and 29.0% forGG , 47.3% forAG , and 23.7% forAA in the controls, and there were statistical differences between the cases and the controls (P <0.05). Compared withGG genotype,AG orAA genotype was significantly associated with an increased risk of cervical cancer (P <0.05), and theOR was 2.38 for individuals withAA genotype versus those withGG (95%CI : 1.17-4.83). No significant association was found between gene polymorphism of miR-196a2 rs11614913 and cervical cancer (P >0.05).Conclusion The miR-30c rs928508 polymorphism may be correlated with cervical cancer.
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