张博, 梅勇, 邹晓雪, 宋冠仪, 刘佩佩, 姬晓宇, 商律. 顶空固相微萃取-气相色谱法测定尿中五氯酚[J]. 环境与职业医学, 2016, 33(12): 1187-1190. DOI: 10.13213/j.cnki.jeom.2016.16342
引用本文: 张博, 梅勇, 邹晓雪, 宋冠仪, 刘佩佩, 姬晓宇, 商律. 顶空固相微萃取-气相色谱法测定尿中五氯酚[J]. 环境与职业医学, 2016, 33(12): 1187-1190. DOI: 10.13213/j.cnki.jeom.2016.16342
ZHANG Bo, MEI Yong, ZOU Xiao-xue, SONG Guan-yi, LIU Peipei, JI Xiao-yu, SHANG Lü. Determination of Pentachlorophenol in Human Urine by Headspace Solid Phase Microextraction Coupled with Gas Chromatography[J]. Journal of Environmental and Occupational Medicine, 2016, 33(12): 1187-1190. DOI: 10.13213/j.cnki.jeom.2016.16342
Citation: ZHANG Bo, MEI Yong, ZOU Xiao-xue, SONG Guan-yi, LIU Peipei, JI Xiao-yu, SHANG Lü. Determination of Pentachlorophenol in Human Urine by Headspace Solid Phase Microextraction Coupled with Gas Chromatography[J]. Journal of Environmental and Occupational Medicine, 2016, 33(12): 1187-1190. DOI: 10.13213/j.cnki.jeom.2016.16342

顶空固相微萃取-气相色谱法测定尿中五氯酚

Determination of Pentachlorophenol in Human Urine by Headspace Solid Phase Microextraction Coupled with Gas Chromatography

  • 摘要: 目的

    建立顶空固相微萃取-气相色谱法测定尿中五氯酚的方法。

    方法

    利用顶空固相微萃取技术,富集尿中五氯酚,优化萃取条件和色谱柱,利用电子捕获检测器测定。

    结果

    该方法测定范围为0.50~5.00 mg/L,相关系数r=0.999 3,检出限为0.09 mg/L,平均回收率为97.6%~103.7%,精密度为1.63%~6.78%。

    结论

    顶空固相微萃取技术处理尿样简便快速,检出限低,结果准确可靠,可用于职业接触五氯酚人群尿中五氯酚的测定。

     

    Abstract: Objective

    To establish a method for pentachlorophenol determination in human urine by headspace solid phase microextraction coupled with gas chromatography.

    Methods

    Headspace solid phase microextraction technology was used to enrich pentachlorophenol in urine.The extraction conditions and the chromatographic column were optimized.Pentachlorophenol was determined with electron capture detector.

    Results

    The linear dection rang for pentachlorophenol in urine was 0.50-5.00 mg/L;the correlation coefficient was 0.999 3;and the limit of detection was 0.09 mg/L.The average recovery rate for pentachlorophenol was from 97.6% to 103.7%,with relative standard deviations of 1.63%-6.78%.

    Conclusion

    The method is featured by easy sample preparation,low limit of detection,and high accuracy,and suitable for the determination of pentachlorophenol in worker's urine.

     

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