宋瑞瑞, 张晓雪, 时亚欣, 王晓艳, 李小明, 李清钊, 沈福海, 蒋守芳, 金玉兰. 3-甲基腺嘌呤对染矽尘大鼠肺组织胶原纤维mRNA表达的影响[J]. 环境与职业医学, 2016, 33(10): 1000-1004. DOI: 10.13213/j.cnki.jeom.2016.16246
引用本文: 宋瑞瑞, 张晓雪, 时亚欣, 王晓艳, 李小明, 李清钊, 沈福海, 蒋守芳, 金玉兰. 3-甲基腺嘌呤对染矽尘大鼠肺组织胶原纤维mRNA表达的影响[J]. 环境与职业医学, 2016, 33(10): 1000-1004. DOI: 10.13213/j.cnki.jeom.2016.16246
SONG Rui-rui, ZHANG Xiao-xue, SHI Ya-xin, WANG Xiao-yan, LI Xiao-ming, LI Qingzhao, SHEN Fu-hai, JIANG Shou-fang, JIN Yu-lan. Impact of 3-Methyladenine on Collagen Fiber mRNA Expression in Lung Tissue of Rat Exposed to Silica Dust[J]. Journal of Environmental and Occupational Medicine, 2016, 33(10): 1000-1004. DOI: 10.13213/j.cnki.jeom.2016.16246
Citation: SONG Rui-rui, ZHANG Xiao-xue, SHI Ya-xin, WANG Xiao-yan, LI Xiao-ming, LI Qingzhao, SHEN Fu-hai, JIANG Shou-fang, JIN Yu-lan. Impact of 3-Methyladenine on Collagen Fiber mRNA Expression in Lung Tissue of Rat Exposed to Silica Dust[J]. Journal of Environmental and Occupational Medicine, 2016, 33(10): 1000-1004. DOI: 10.13213/j.cnki.jeom.2016.16246

3-甲基腺嘌呤对染矽尘大鼠肺组织胶原纤维mRNA表达的影响

Impact of 3-Methyladenine on Collagen Fiber mRNA Expression in Lung Tissue of Rat Exposed to Silica Dust

  • 摘要: 目的

    探讨3-甲基腺嘌呤(3-MA)对染矽尘大鼠肺组织胶原纤维mRNA表达的影响。

    方法

    80只健康雄性大鼠建立矽肺模型,随机分为对照组和3-MA组,3-MA组腹腔注射1.5 mg/(kg·d)3-MA,隔天给药持续28 d,染尘不同时间处死,HE染色观察大鼠肺组织病理组织学变化,测定肺组织中羟脯氨酸、Ⅰ型和Ⅲ型胶原纤维mRNA含量。

    结果

    HE染色显示,3-MA组每个时间点肺组织纤维化程度均比对照组轻,随着染尘时间延长肺组织纤维化有不同程度的改变。3-MA组大鼠7 d(0.39±0.02)、14 d(0.43±0.01)、28 d(0.36±0.02)、60 d(0.34±0.04)、90 d(0.49±0.03)肺组织羟脯氨酸含量均低于同一时间点对照组大鼠(0.41±0.03,0.48±0.01,0.49±0.02,0.60±03.02,0.73±0.03)(P < 0.05),不同时间点羟脯氨酸含量差异有统计学意义(F=55.99,P < 0.05)。3-MA组7 d肺组织Ⅰ型胶原纤维mRNA含量(1.11±0.30)比对照组(0.83±0.13)高,除28 d外其余各时间点(14 d:0.70±0.23,60 d:0.92±0.08,90 d:1.38±0.37)均低于对照组(14 d:1.06±0.16,60 d:1.10±0.25,90 d:2.06±0.66)(P < 0.05),不同时间点其差异有统计学意义(H=23.36,P < 0.05)。3-MA组7 d肺组织Ⅲ型胶原mRNA含量较高(0.77±0.19),在14 d(0.44±0.26)、28 d(0.35±0.09)及60 d(0.31±0.06)时呈现下降趋势,90 d(1.44±0.31)时又再度升高,各时间点差异有统计学意义(H=28.77,P < 0.05),各时间点其含量均低于对照组(7 d:0.83±0.13,14 d:1.06±0.16,28 d:0.98±0.11,60 d:1.10±0.25,90 d:2.06±0.67)(P < 0.05)。

    结论

    3-MA干预后,与对照组相比同一时间点及同一组不同时间大鼠肺组织胶原纤维mRNA表达不同,影响大鼠矽肺纤维化发生发展进程。

     

    Abstract: Objective

    To examine the collagen fiber mRNA expression in rat lung tissue exposed to silica dust impacted by 3-methyladenine (3-MA).

    Methods

    Eighty healthy male rats were developed silicosis and randomly divided into a silicosis control group and a 3-MA group. The 3-MA group received intraperitoneal injection of 1.5 mg/(kg·d) 3-MA every other day for 28 days. Rats were executed at different time points to observe pathologic changes of rat lung tissue by HE staining and to measure the mRNA levels of hydroxyproline, collagen type Ⅰ fiber, and collagen type Ⅲ fiber.

    Results

    By HE staining, the degree of lung fibrosis in the 3-MA group was lighter than that of the control group in every selected time point. The lung tissue samples showed different fibrosis degrees along with prolonged dust exposure. The hydroxyproline levels in the 3-MA group at 7 d (0.39±0.02), 14 d (0.43±0.01), 28 d (0.36±0.02), 60 d (0.34±0.04) and 90 d (0.49±0.03) were lower than those in the control group at corresponding time points (0.41±0.03, 0.48±0.01, 0.49±0.02, 0.60±03.02, 0.73±0.03) (P < 0.05), and the difference among different time points was statistically significant (F=55.99, P < 0.05). The collagen type Ⅰ fiber mRNA level of the 3-MA group at 7 d (1.11±0.30) was higher than that of the control group (0.83±0.13), and except of 28 d, the levels at other time points (14 d: 0.70±0.23, 60 d: 0.92±0.08, 90 d: 1.38±0.37) were lower than those of the control group (14 d: 1.06±0.16, 60 d: 1.10±0.25, 90 d: 2.06±0.66) (P < 0.05), the statistical differences among different time points in same group were identified (H=23.36, P < 0.05). The collagen type Ⅲ fiber mRNA level of the 3-MA group was (0.77±0.19) at 7 d, declined at 14 d (0.44±0.26), 28 d (0.35±0.09), and 60 d (0.31±0.06), and rose again at 90 d (1.44±0.31), showing significant differences among time points in same group (H=28.77, P < 0.05); moreover, the collagen typeⅢfiber mRNA levels of the 3-MA group were lower than those of the control group at each time point (7 d: 0.83±0.13, 14 d: 1.06±0.16, 28 d: 0.98±0.11, 60 d: 1.10±0.25, 90 d: 2.06±0.67) (P < 0.05).

    Conclusion

    After the intervention of 3-MA, the collagen fiber mRNA expression levels are different compared with the control group at the same time point or the same group at different time points. It can be concluded that 3-MA may affect the development of silicosis fibrosis in rats.

     

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