微小RNA-125b对肺癌细胞95D生物学功能的影响
Effect on Biological Function of Lung Cancer 95D Cells by MicroRNA-125b
-
摘要:
目的 探讨微小RNA(miR)-125b对肺癌细胞95D生物学功能的影响。
方法 以95D细胞为实验对象,分别转染miR-125b模拟物和模拟物阴性对照,未经特殊处理为空白组。应用MTT法、流式细胞仪和Transwell小室法分别检测miR-125b表达改变对95D细胞增殖、凋亡、周期及侵袭的影响。
结果 95D细胞转染48 h后,转染组miR-125b的水平提高,表达量为阴性对照组的22.32倍。与阴性对照组相比,miR-125b模拟物转染组细胞凋亡率明显降低(13.77±0.52)%,(9.90±1.33)%,
P < 0.05,细胞增殖、周期、侵袭无显著改变(P > 0.05)。结论 miR-125b基因过表达能够抑制肺癌95D细胞凋亡。
Abstract:Objective To investigate the effect of microRNA (miR)-125b on biological function of lung cancer 95D cells.
Methods The study transfected miR-125b mimics into 95D cells (mimics transfection group). A mimics negative control group and a blank group were also established. MTT assay, flow cytometry, and Transwell assay were used to examine the effects of up-regulated miR-125b on the proliferation, apoptosis, cycle, and invasive ability of 95D cells.
Results After 48 h of transfection, the level of miR-125b was increased significantly in the mimics transfection group, which was 22.32 times of the negative control group. The apoptosis rate of 95D cells in the miR-125b mimics transfection group(9.90±1.33)% was significantly lower than that in the negative control group(13.77±0.52)% (
P < 0.05), but no significant changes were found in cell proliferation, cycle, and invasive ability (P >0.05).Conclusion Over-expression of miR-125b could inhibit the apoptosis of lung cancer 95D cells.